TAR DNA Binding Protein (TARDBP) (Internal Region) antibody

Details for Product No. ABIN334503
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Antigen
Synonyms TARDBP, TDP43, DKFZp459I2127, tardbp, ALS10, TDP-43, 1190002A23Rik, C85084, Tdp43, wu:fb77f02, wu:fc52g10
Epitope
Internal Region
(23), (22), (15), (13), (7), (7), (7), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(123), (55), (48), (23), (16), (8), (7), (6), (5), (3), (1), (1)
Host
Goat
(95), (30), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(113), (47), (35), (28), (27), (21), (10), (9), (5), (4), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN334503
374.27 $
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Immunogen Peptide with sequence C-HISNAEPKHNSNRQ, from the internal region of the protein sequence according to NP_031401.1.
Cross-Reactivity Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name TARDBP
Background Synonyms: TARDBP, TAR DNA binding protein, ALS10, TDP-43, OTTHUMP00000002171, TAR DNA-binding protein-43
Gene ID 23435, 230908
NCBI Accession NP_031401
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 45 kDa band observed in lysates of cell lines Jurkat and NIH3T3 (calculated MW of 44.7 kDa according to NP_031401.1). A slightly larger protein is detected in some regions of the brain. Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-TAR DNA Binding Protein (TARDBP) (Internal Region) antibody ABIN334503 (0.3 ug/ml) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zhang, Xu, Dickey et al.: "Progranulin mediates caspase-dependent cleavage of TAR DNA binding protein-43." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 39, pp. 10530-4, 2007 (PubMed).

Validation Images
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