WAS/WASL Interacting Protein Family, Member 1 (WIPF1) antibody

Antigen: WAS/WASL Interacting Protein Family, Member 1 (WIPF1)

Synonyms: WIP, PRPL-2, WASPIP, MGC111041, Wip, Waspip, AI115543, D2Ertd120e

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334525

Additional Information

Alternative name: WIPF1

Gene ID: 7456, 215280 (mouse), 117538 (rat)

Immunogen: C-KLARNESRSGSNRRE

Description: All names and symbols: WIPF1, WAS/WASL interacting protein family, member 1 , MGC111041, PRPL-2, WASPIP, WIP, Wiskott-Aldrich syndrome protein interacting protein. Official Symbol: WIPF1. RefSeq number(s): NP_003378.3, NP_001070737.1

Specificity: Both variants represent identical proteins Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 55kDa band observed in Human Lymph Node, Spleen and Tonsil lysates (calculated MW of 51.3kDa according to NP_003378.3 and NP_001070737.1). Recommended concentration: 0.03-0.1

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Images

ABIN334525 (0.03 ug/ml) staining of Human Tonsil lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Massarwa, Carmon, Shilo et al.: "WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila." in: Developmental cell, Vol. 12, Issue 4, pp. 557-69, 2007 (PubMed).