Rubisco Large Subunit, Form I and Form II antibody

Antigen: Rubisco Large Subunit, Form I and Form II

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii (C. reinhardtii), Cyanophora paradoxa, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Guzmania hybrid, Micromonas pusilla, Moss (Physcomitrella patens), Red algae (Porphyra), Spinach (Spinacia oleracea), Synechococcus PCC 7942, Thalassiosira pseudonana, Prochlorococcus

Application: Western Blotting (WB), Immunofluorescence (IF), Confocal Microscopy (CM), Immunolocalization (IL)

Catalog no.: ABIN334533

Additional Information

Alternative name: Rubisco large subunit, form I and form II (RbcL)

Immunogen: KLH-conjugated synthetic peptide conserved across all known plant, algal and(cyano)bacterial RbcL protein sequences (form I L8S8 and form II L2), includingArabidopsis thaliana AtCg00490, Hordeum vulgare P05698, Oryza sativaP0C510, Chlamydomonas reinhardtii P00877, Synechococcus PCC 7920A5CKC5

Format: Lyophilized

Description: This antibody is especially suitable for quantifying of Rubisco in plant andalgal samples. Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes therate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundantproteins on Earth and its homology has been demonstrated from purple bacteriato flowering plants.

Specificity: Predicted reactivity: di and monocots,conifers,mosses, liverworts, welwitschia, green algae, red alge,brown algae, cryptomonad, cyanobacteria including prochlorophytes,gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria.

Application Details

Application Notes: Recommended Dilution 1: 5000 -10 000 with standard ECL (WB), immunofluorescence/confocalmicroscopy (IF), 1: 250 images in Prins et al. 2998, detailed protocol available onrequest (IL). Additional Information: antibody is a cellular [compartment marker] of plastid stroma or a cytopasmmarker in cyanobacteria. Antibody can detect Rubisco protein from 31.25 fmoles.Antibody will detect Rubisco Form I and Form II, therefore is suitable for workwith samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms,Raphidophytes, brown algae) as well as higher plants. This antibody can be usedto quantify Rubisco protein in plant and algal samples.

Buffer: Affinity purified serum in PBS pH 7.4

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Ifuku, Yamamoto, Ono et al.: "PsbP protein, but not PsbQ protein, is essential for the regulation and stabilization of photosystem II in higher plants." in: Plant physiology, Vol. 139, Issue 3, pp. 1175-84, 2005 (PubMed).

Oesterhelt, Schmaelzlin, Schmitt et al.: "Regulation of photosynthesis in the unicellular acidophilic red alga Galdieria sulphuraria." in: The Plant journal : for cell and molecular biology, Vol. 51, Issue 3, pp. 500-11, 2007 (PubMed).

Li, Xing, Guo et al.: "Drought tolerance through over-expression of the expansin gene TaEXPB23 in transgenic tobacco." in: Journal of plant physiology, 2011 (PubMed).