Ubiquitin (UBQ11) antibody

Antigen: Ubiquitin (UBQ11)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Barley (Hordeum vulgare), Nicotiana benthamiana, Tomato (Solanum lycopersicum)

Application: Western Blotting (WB)

Catalog no.: ABIN334561

Additional Information

Immunogen: recombinant Arabidopsis thaliana ubiquitin, ubq11, with N-terminal His-tag, locusaccession: At4g05050

Format: Lyophilized

Description: Ubiquitin is a highly conserved regulatory protein expressed in all eukaryotictissues. Originally this protein was called: Ubiquitous Immunopoietic Polypeptide.Its function is labeling of proteins for degradation through ubiquitin proteasomesystem (UPS).

Specificity: Predicted reactivity: dicots including: Nicotiana tabacum, Glycine max, monocots including: Hordeumvulgare, Oryza sativa, Zea mays. Not reactive in: algae.

Application Details

Application Notes: Recommended Dilution 1: 10 000 with standard ECL (WB).

Purity: Serum

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Images

5 μg of recombinant protein from Phytophora sp. (1), human (2), Arabidopsis thaliana His-tagged ubiquitin (3), Arabidopsis thaliana His-tagged SUMO protein (4), was separated on 15% PAA gel and blotted on PVDF membrane. Filters were blocked in 5% milk for 1h, incubated with 1: 10 000 anti-ubiquitin antibody (1h), followed by incubation with 1: 15 000 secondary anti-rabbit antibodies(1h) coupled with HRP and visualization (10 seconds exposure) with standard ECL.