Nitroso-L.glutathione (GSNO) antibody

Antigen: Nitroso-L.glutathione (GSNO)

Clonality: Polyclonal

Host: Rat

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Catalog no.: ABIN334580

Additional Information

Immunogen: synthetic Nitroso-L.Glutathione conjugated to protein carrier (PC)

Format: Lyophilized

Description: GSNO, S-nitrosoglutathione belongs to a family of molecules involved innitrosative stress. GSNO is a natural source of nitric oxide (NO). S-nitrosylationreaction of NO with GSH leads to formation of S-nitroso glutathione (GSNO).Selected references: Barroso et al (2006). Localization of S-nitrosoglutathione andexpression of S-nitrosoglutatione reductase in pea plants under cadmium stress. J Exp Bot. 2006,57(8):1785-93. Valderrama et al (2007). Nitrosative stress inplants. FEBS Lett. 6,581(3):453-6. Corpas et al (2008). Localization ofS-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathionereductase activity in plants. Methods Enzymol. 437:561-74

Specificity: GSNO nitroso-L.glutathione. Predicted reactivity: GSNO nitroso-L.glutathione.

Application Details

Application Notes: Recommended Dilution 1: 1000 - 1: 2000 (WB), 1: 1000 - 1: 5000 (ICH), 1: 5000 - 1: 10 000 (ELISA).

Buffer: Total IgG in PBS, pH 7.35

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Kangasjaervi, Lepistoe, Haennikaeinen et al.: "Diverse roles for chloroplast stromal and thylakoid-bound ascorbate peroxidases in plant stress responses." in: The Biochemical journal, Vol. 412, Issue 2, pp. 275-85, 2008 (PubMed).

Hideg, Kuos, Schreiber: "Imaging of NPQ and ROS formation in tobacco leaves: heat inactivation of the water-water cycle prevents down-regulation of PSII." in: Plant & cell physiology, Vol. 49, Issue 12, pp. 1879-86, 2008 (PubMed).