Red Fluorescent Protein (RFP) antibody

Details for Product No. ABIN334653
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Synonyms RFP, RNF76
(25), (9), (4), (1), (1)
(20), (15), (5)
Clonality (Clone)
Monoclonal ()
(4), (2), (2), (1)
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
(36), (29), (9), (5), (4), (4), (4), (2), (1)
Pubmed 31 references available
Quantity 100 µg
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Catalog No. ABIN334653
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 5F8
Isotype IgG2a
Specificity 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensitivity Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Characteristics Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Alternative Name RFP
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Restrictions For Research Use only
Format Liquid
Concentration 1mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
anti-Red Fluorescent Protein (RFP) antibody primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe) detection with ECL Western Blot Detection Kit
anti-Red Fluorescent Protein (RFP) antibody (2) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of approximately 72 kDa (predicted molecular weight of RFP-PCNA: 72 kDa). Primary antibody: 0.2 µg of RFP antibody 5F8 incubation for 1 h at RT, secondary antibody: anti-rat-Alexa647
anti-Red Fluorescent Protein (RFP) antibody (3) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
anti-Red Fluorescent Protein (RFP) antibody (4) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation for 1 h at RT. A 50 kDa band is precipitated which is IgG heavy chain whilst the 30 kDa band is PCNA.
anti-Red Fluorescent Protein (RFP) antibody (5) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primary antibody: 1/1000 RFP antibody 5F8 incubation for 1 h at RT. Secondary antibody: 1/400 donkey-anti-rat-Alexa488 incubation for 1 h at RT
Product cited in: Tags/Labels: Rancz, Franks, Schwarz et al.: "Transfection via whole-cell recording in vivo: bridging single-cell physiology, genetics and connectomics." in: Nature neuroscience, Vol. 14, Issue 4, pp. 527-32, 2011 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (1:1000).

Tønnesen, Parish, Sørensen et al.: "Functional integration of grafted neural stem cell-derived dopaminergic neurons monitored by optogenetics in an in vitro Parkinson model." in: PLoS ONE, Vol. 6, Issue 3, pp. e17560, 2011 (PubMed). (1:1000). Further details: Slices were incubated 15 h

Graewe, Rankin, Lehmann et al.: "Hostile Takeover by Plasmodium: Reorganization of Parasite and Host Cell Membranes during Liver Stage Egress." in: PLoS pathogens, Vol. 7, Issue 9, pp. e1002224, 2011 (PubMed).

Uhrig, Moorhead: "Two ancient bacterial-like PPP family phosphatases from Arabidopsis are highly conserved plant proteins that possess unique properties." in: Plant physiology, Vol. 157, Issue 4, pp. 1778-92, 2011 (PubMed).

Petrovská, Cenklová, Pochylová et al.: "Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication." in: The New phytologist, Vol. 193, Issue 3, pp. 590-604, 2012 (PubMed).

Yoshihara, Takahashi, Nishimura et al.: "5T4 Glycoprotein Regulates the Sensory Input-Dependent Development of a Specific Subtype of Newborn Interneurons in the Mouse Olfactory Bulb." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 6, pp. 2217-2226, 2012 (PubMed). (1:1000).

Ingmundson, Nahar, Brinkmann et al.: "The exported Plasmodium berghei protein IBIS1 delineates membranous structures in infected red blood cells." in: Molecular microbiology, 2012 (PubMed). (1:1000).

Mu, Berti, Masserdotti et al.: "SoxC Transcription Factors Are Required for Neuronal Differentiation in Adult Hippocampal Neurogenesis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 9, pp. 3067-3080, 2012 (PubMed). (1:300).

Shimada, LeComte, Granger et al.: "Self-renewal and differentiation of reactive astrocyte-derived neural stem/progenitor cells isolated from the cortical peri-infarct area after stroke." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 23, pp. 7926-40, 2012 (PubMed).

Pathania, Torres-Reveron, Yan et al.: "miR-132 enhances dendritic morphogenesis, spine density, synaptic integration, and survival of newborn olfactory bulb neurons." in: PLoS ONE, Vol. 7, Issue 5, pp. e38174, 2012 (PubMed).

Piotrowska-Nitsche, Caspary: "Live imaging of individual cell divisions in mouse neuroepithelium shows asymmetry in cilium formation and Sonic hedgehog response." in: Cilia, Vol. 1, Issue 1, 2013 (PubMed).

Schoberer, Liebminger, Botchway et al.: "Time-resolved fluorescence imaging reveals differential interactions of N-glycan processing enzymes across the Golgi stack in planta." in: Plant physiology, Vol. 161, Issue 4, pp. 1737-54, 2013 (PubMed). (1:2000).

Tanji, Nishikori, Shiraishi et al.: "Co-operative function and mutual stabilization of the half ATP-binding cassette transporters HAF-4 and HAF-9 in Caenorhabditis elegans." in: The Biochemical journal, Vol. 452, Issue 3, pp. 467-75, 2013 (PubMed).

Li, Roszko, Sepich et al.: "Gpr125 modulates Dishevelled distribution and planar cell polarity signaling." in: Development (Cambridge, England), Vol. 140, Issue 14, pp. 3028-39, 2013 (PubMed).

Viganò, Möbius, Götz et al.: "Transplantation reveals regional differences in oligodendrocyte differentiation in the adult brain." in: Nature neuroscience, Vol. 16, Issue 10, pp. 1370-2, 2013 (PubMed). (1:500).

Jedrusik-Bode, Studencka, Smolka et al.: "The sirtuin SIRT6 regulates stress granule formation in C. elegans and mammals." in: Journal of cell science, Vol. 126, Issue Pt 22, pp. 5166-77, 2013 (PubMed).

Berndt, Wilkinson, Heimann et al.: "In vivo characterisation of the properties of SUMO1-specific monobodies." in: The Biochemical journal, 2013 (PubMed).

Matz, Matuschewski, Kooij: "Two putative protein export regulators promote Plasmodium blood stage development in vivo." in: Molecular and biochemical parasitology, Vol. 191, Issue 1, pp. 44-52, 2013 (PubMed).

Li, Stewart, Canepari et al.: "Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 4, pp. 1280-92, 2014 (PubMed). Method employed by authors: Immunohistochemistry (IHC) Further details: When immunohistochemistry was performed on sections of nNOS-Cre-tdTomato mice, resectioned slices (50 μm) were incubated with a primary antibody raised against nNOS and tdTomato (1:500 Rat RFP 5F8) overnight at 4°C.

Kallenberger, Beaudouin, Claus et al.: "Intra- and Interdimeric Caspase-8 Self-Cleavage Controls Strength and Timing of CD95-Induced Apoptosis." in: Science signaling, Vol. 7, Issue 316, pp. ra23, 2014 (PubMed).

Jahnel, Walzl, Technau: "Development and epithelial organisation of muscle cells in the sea anemone Nematostella vectensis." in: Frontiers in zoology, Vol. 11, pp. 44, 2014 (PubMed).

General Rottach, Kremmer, Nowak et al.: "Generation and characterization of a rat monoclonal antibody specific for multiple red fluorescent proteins." in: Hybridoma (2005), Vol. 27, Issue 5, pp. 337-43, 2008 (PubMed).

Bakondi, Spees: "Human CD133-derived bone marrow stromal cells establish ectopic hematopoietic microenvironments in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 400, Issue 2, pp. 212-8, 2010 (PubMed).

Laxman, Sutter, Tu: "Behavior of a metabolic cycling population at the single cell level as visualized by fluorescent gene expression reporters." in: PLoS ONE, Vol. 5, Issue 9, pp. e12595, 2010 (PubMed).

Korzeniowski, Manjarrés, Varnai et al.: "Activation of STIM1-Orai1 involves an intramolecular switching mechanism." in: Science signaling, Vol. 3, Issue 148, pp. ra82, 2010 (PubMed).

Qin, Leonhardt, Spada: "Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1." in: Journal of cellular biochemistry, 2010 (PubMed).

Goldmann, Overlack, Wolfrum et al.: "PTC124 mediated translational read-through of a nonsense mutation causing Usher type 1C." in: Human gene therapy, 2011 (PubMed).

Chuhma, Tanaka, Hen et al.: "Functional connectome of the striatal medium spiny neuron." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 4, pp. 1183-92, 2011 (PubMed).

Speck, Arndt, Ma: "Efficient phage display of intracellularly folded proteins mediated by the TAT pathway." in: Protein engineering, design & selection : PEDS, 2011 (PubMed).

Frauer, Rottach, Meilinger et al.: "Different Binding Properties and Function of CXXC Zinc Finger Domains in Dnmt1 and Tet1." in: PLoS ONE, Vol. 6, Issue 2, pp. e16627, 2011 (PubMed).

Pichler, Jack, Wolf et al.: "Versatile toolbox for high throughput biochemical and functional studies with fluorescent fusion proteins." in: PLoS ONE, Vol. 7, Issue 5, pp. e36967, 2012 (PubMed).

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