anti-RFP antibody (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN334653
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Antigen
Synonyms RFP, RNF76
Reactivity
Discosoma
(33), (9), (4), (2), (1), (1)
Host
Rat
(23), (22), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is un-conjugated
(4), (2), (2), (1)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(46), (33), (12), (10), (8), (5), (4), (4), (1)
Pubmed 21 references available
Quantity 100 μL
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 5F8
Isotype IgG2a
Specificity 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensitivity Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Characteristics Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Alternative Name RFP (RFP Antibody Abstract)
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Restrictions For Research Use only
Format Liquid
Concentration 1mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
anti-Red Fluorescent Protein (RFP) antibody primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary...
anti-Red Fluorescent Protein (RFP) antibody (2) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of ...
anti-Red Fluorescent Protein (RFP) antibody (3) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
anti-Red Fluorescent Protein (RFP) antibody (4) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation f...
anti-Red Fluorescent Protein (RFP) antibody (5) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primar...
Product cited in: Loriol, Cassé, Khayachi et al.: "mGlu5 receptors regulate synaptic sumoylation via a transient PKC-dependent diffusional trapping of Ubc9 into spines." in: Nature communications, Vol. 5, pp. 5113, 2014 (PubMed).

Thomas, Cantagrel, Mariani et al.: "Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity." in: European journal of human genetics : EJHG, 2014 (PubMed).

Yoshihara, Takahashi, Nishimura et al.: "Npas4 regulates Mdm2 and thus Dcx in experience-dependent dendritic spine development of newborn olfactory bulb interneurons." in: Cell reports, Vol. 8, Issue 3, pp. 843-57, 2014 (PubMed).

Xue, Atallah, Scanziani: "Equalizing excitation-inhibition ratios across visual cortical neurons." in: Nature, Vol. 511, Issue 7511, pp. 596-600, 2014 (PubMed).

Daido, Hamanishi, Kusakabe: "Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: implications for photoreceptor subtype specification." in: Developmental biology, Vol. 392, Issue 1, pp. 117-29, 2014 (PubMed).

Haussig, Matuschewski, Kooij: "Identification of vital and dispensable sulfur utilization factors in the Plasmodium apicoplast." in: PLoS ONE, Vol. 9, Issue 2, pp. e89718, 2014 (PubMed).

Ledri, Madsen, Nikitidou et al.: "Global optogenetic activation of inhibitory interneurons during epileptiform activity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 9, pp. 3364-77, 2014 (PubMed).

Engelstoft, Park, Sakata et al.: "Seven transmembrane G protein-coupled receptor repertoire of gastric ghrelin cells." in: Molecular metabolism, Vol. 2, Issue 4, pp. 376-92, 2013 (PubMed).

Kenthirapalan, Waters, Matuschewski et al.: "Copper-transporting ATPase is important for malaria parasite fertility." in: Molecular microbiology, Vol. 91, Issue 2, pp. 315-25, 2014 (PubMed).

Heiber, Kruse, Pick et al.: "Identification of new PNEPs indicates a substantial non-PEXEL exportome and underpins common features in Plasmodium falciparum protein export." in: PLoS pathogens, Vol. 9, Issue 8, pp. e1003546, 2013 (PubMed).

Arguello, Yang, Vogt et al.: "Dapper antagonist of catenin-1 cooperates with Dishevelled-1 during postsynaptic development in mouse forebrain GABAergic interneurons." in: PLoS ONE, Vol. 8, Issue 6, pp. e67679, 2013 (PubMed).

Marthiens, Rujano, Pennetier et al.: "Centrosome amplification causes microcephaly." in: Nature cell biology, Vol. 15, Issue 7, pp. 731-40, 2013 (PubMed).

Chen, Vogt, Wang et al.: "Use of "MGE enhancers" for labeling and selection of embryonic stem cell-derived medial ganglionic eminence (MGE) progenitors and neurons." in: PLoS ONE, Vol. 8, Issue 5, pp. e61956, 2013 (PubMed).

Stahl, Walcher, De Juan Romero et al.: "Trnp1 regulates expansion and folding of the mammalian cerebral cortex by control of radial glial fate." in: Cell, Vol. 153, Issue 3, pp. 535-49, 2013 (PubMed).

Roloff, Spillner, Kehlenbach: "Several phenylalanine-glycine motives in the nucleoporin Nup214 are essential for binding of the nuclear export receptor CRM1." in: The Journal of biological chemistry, Vol. 288, Issue 6, pp. 3952-63, 2013 (PubMed).

Orito, Ishino, Iwanaga et al.: "Liver-specific protein 2: a Plasmodium protein exported to the hepatocyte cytoplasm and required for merozoite formation." in: Molecular microbiology, Vol. 87, Issue 1, pp. 66-79, 2013 (PubMed).

Grüring, Heiber, Kruse et al.: "Uncovering common principles in protein export of malaria parasites." in: Cell host & microbe, Vol. 12, Issue 5, pp. 717-29, 2012 (PubMed).

Kenthirapalan, Waters, Matuschewski et al.: "Flow cytometry-assisted rapid isolation of recombinant Plasmodium berghei parasites exemplified by functional analysis of aquaglyceroporin." in: International journal for parasitology, Vol. 42, Issue 13-14, pp. 1185-92, 2012 (PubMed).

Goldmann, Overlack, Möller et al.: "A comparative evaluation of NB30, NB54 and PTC124 in translational read-through efficacy for treatment of an USH1C nonsense mutation." in: EMBO molecular medicine, Vol. 4, Issue 11, pp. 1186-99, 2012 (PubMed).

Young, Taylor, Wu et al.: "NKCC1 knockdown decreases neuron production through GABA(A)-regulated neural progenitor proliferation and delays dendrite development." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 39, pp. 13630-8, 2012 (PubMed).

Luo, Chen, Yue et al.: "Old flies have a robust central oscillator but weaker behavioral rhythms that can be improved by genetic and environmental manipulations." in: Aging cell, Vol. 11, Issue 3, pp. 428-38, 2012 (PubMed).

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Catalog No. ABIN334653
254.38 $
Plus shipping costs $45.00
Quantity
Price
100 μL
254.38 $

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