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Red Fluorescent Protein (RFP) antibody
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
|20 references available|
|Price||304.92 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Description||Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc) which become attractive tools for a number of applications. For example the combination of green and red fusion proteins allows multicolor tracking as well as protein-protein interaction studies in living cells. Here we offer a rat monoclonal antibody (MAb) against multiple red FPs, designated as multi-red 5F8. The 5F8 MAb is characterized by high affinity and specificity against the DsRed derivatives and corresponding fusion proteins. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays. With this versatile antibody red fluorescent proteins and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or enzymatic analyses as well as for microscopic analyses.|
|Specificity||5F8 recognizes various Red fluorescent proteins derived from DsRed and RFP-tagged fusion proteins.|
|Application Notes||Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight RFP: 30 kDa). Immunoprecipitation: For immunoprecipitation 4ug of multi-red antibody 5F8 used incubation for 1 h at RT. A 50 kDa band is precipitated which is IgG heavy chain whilst the 30kDa band is PCNA.Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3,7% PFA fixed. Used at a dilution of 1/1000. Secondary antibody: donkey-anti-rat-Alexa488 (1/400)|
|Purification||hybridoma supernatant. The hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml.|
|Preservative||0.01% Sodium Azide|
|Storage||Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C. Store at +4 degree C short term (1-2 weeks). Avoid freeze / thaw cycles.|
|Restrictions||For Research Use only|
|primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe) detection with ECL Western Blot Detection Kit|
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|Hosts||Rabbit (13), Mouse (5), Chicken (4)|
|Reactivities||Discosoma (5), Tag (2), All Species (1), Sea Anemone (1), tag (1)|
|Applications||Western Blotting (WB) (18), ELISA (15), Immunohistochemistry (IHC) (6), Immunoprecipitation (IP) (4), Fluorescence Microscopy (FM) (3), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Immunofluorescence (IF) (2), Immunostaining (ISt) (1)|
|Conjugates||Biotin (2), FITC (2), HRP (2)|
|Epitopes||AA 234 (11), N-Term (2)|