anti-RFP antibody (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN334653
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Antigen
Synonyms RFP, RNF76
Reactivity
Discosoma
(33), (11), (5), (2), (1), (1)
Host
Rat
(24), (24), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
This RFP antibody is un-conjugated
(4), (2), (2), (1)
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(49), (35), (12), (11), (8), (5), (2), (2), (1)
Pubmed 21 references available
Quantity 100 μL
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 5F8
Isotype IgG2a
Specificity 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensitivity Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Characteristics Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Alternative Name RFP (RFP Antibody Abstract)
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Restrictions For Research Use only
Format Liquid
Concentration 1mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
anti-Red Fluorescent Protein (RFP) antibody primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary...
anti-Red Fluorescent Protein (RFP) antibody (2) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of ...
anti-Red Fluorescent Protein (RFP) antibody (3) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
anti-Red Fluorescent Protein (RFP) antibody (4) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation f...
anti-Red Fluorescent Protein (RFP) antibody (5) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primar...
Product cited in: Bhat, Ishitsuka, Inaba et al.: "Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2015 (PubMed).

Krol, Krol, Alvarez et al.: "A network comprising short and long noncoding RNAs and RNA helicase controls mouse retina architecture." in: Nature communications, Vol. 6, pp. 7305, 2015 (PubMed).

Miwa, Era: "Generation and characterization of PDGFRα-GFPCreERT2 knock-In mouse line." in: Genesis (New York, N.Y. : 2000), Vol. 53, Issue 5, pp. 329-36, 2015 (PubMed).

Lisak, Schacht, Enders et al.: "The transmembrane Bax inhibitor motif (TMBIM) containing protein family: Tissue expression, intracellular localization and effects on the ER CA(2+)-filling state." in: Biochimica et biophysica acta, Vol. 1853, Issue 9, pp. 2104-14, 2015 (PubMed).

Juris, Montino, Rube et al.: "PI3P binding by Atg21 organises Atg8 lipidation." in: The EMBO journal, Vol. 34, Issue 7, pp. 955-73, 2015 (PubMed).

Petre, Saunders, Sklenar et al.: "Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments." in: Molecular plant-microbe interactions : MPMI, Vol. 28, Issue 6, pp. 689-700, 2015 (PubMed).

Gerstenmaier, Pilla, Herrmann et al.: "The autophagic machinery ensures nonlytic transmission of mycobacteria." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 7, pp. E687-92, 2015 (PubMed).

Nøhr, Egerod, Christiansen et al.: "Expression of the short chain fatty acid receptor GPR41/FFAR3 in autonomic and somatic sensory ganglia." in: Neuroscience, Vol. 290, pp. 126-37, 2015 (PubMed).

Bourane, Grossmann, Britz et al.: "Identification of a spinal circuit for light touch and fine motor control." in: Cell, Vol. 160, Issue 3, pp. 503-15, 2015 (PubMed).

Seeger-Armbruster, Bosch-Bouju, Little et al.: "Patterned, but not tonic, optogenetic stimulation in motor thalamus improves reaching in acute drug-induced Parkinsonian rats." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 3, pp. 1211-6, 2015 (PubMed).

Traenkle, Emele, Anton et al.: "Monitoring interactions and dynamics of endogenous beta-catenin with intracellular nanobodies in living cells." in: Molecular & cellular proteomics : MCP, Vol. 14, Issue 3, pp. 707-23, 2015 (PubMed).

Irie, Weinberger, Tang et al.: "SOX17 is a critical specifier of human primordial germ cell fate." in: Cell, Vol. 160, Issue 1-2, pp. 253-68, 2015 (PubMed).

Fairchild, Smendziuk, Tanentzapf: "A somatic permeability barrier around the germline is essential for Drosophila spermatogenesis." in: Development (Cambridge, England), Vol. 142, Issue 2, pp. 268-81, 2015 (PubMed).

Poças, Branco-Santos, Herrera et al.: "α-Synuclein modifies mutant huntingtin aggregation and neurotoxicity in Drosophila." in: Human molecular genetics, Vol. 24, Issue 7, pp. 1898-907, 2015 (PubMed).

Wetzel, Herrmann, Swapna et al.: "The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite." in: The Journal of biological chemistry, Vol. 290, Issue 3, pp. 1712-28, 2015 (PubMed).

Loriol, Cassé, Khayachi et al.: "mGlu5 receptors regulate synaptic sumoylation via a transient PKC-dependent diffusional trapping of Ubc9 into spines." in: Nature communications, Vol. 5, pp. 5113, 2014 (PubMed).

Thomas, Cantagrel, Mariani et al.: "Identification of a novel ARL13B variant in a Joubert syndrome-affected patient with retinal impairment and obesity." in: European journal of human genetics : EJHG, 2014 (PubMed).

Yoshihara, Takahashi, Nishimura et al.: "Npas4 regulates Mdm2 and thus Dcx in experience-dependent dendritic spine development of newborn olfactory bulb interneurons." in: Cell reports, Vol. 8, Issue 3, pp. 843-57, 2014 (PubMed).

Xue, Atallah, Scanziani: "Equalizing excitation-inhibition ratios across visual cortical neurons." in: Nature, Vol. 511, Issue 7511, pp. 596-600, 2014 (PubMed).

Vázquez-Novelle, Sansregret, Dick et al.: "Cdk1 inactivation terminates mitotic checkpoint surveillance and stabilizes kinetochore attachments in anaphase." in: Current biology : CB, Vol. 24, Issue 6, pp. 638-45, 2014 (PubMed).

Ledri, Madsen, Nikitidou et al.: "Global optogenetic activation of inhibitory interneurons during epileptiform activity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 9, pp. 3364-77, 2014 (PubMed).

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Catalog No. ABIN334653
254.38 $
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Quantity
Price
100 μL
254.38 $

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