Red Fluorescent Protein (RFP) antibody

Details for Product No. ABIN334653
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Antigen
Synonyms RFP, RNF76
Reactivity
Discosoma
(28), (9), (4), (1), (1)
Host
Rat
(20), (18), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (2), (2), (1)
Application
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
(39), (31), (9), (5), (5), (4), (4), (3), (1)
Pubmed 60 references available
Quantity 100 μg
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Catalog No. ABIN334653
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 5F8
Isotype IgG2a
Specificity 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensitivity Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Characteristics Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Purification Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Alternative Name RFP
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Restrictions For Research Use only
Format Liquid
Concentration 1mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
anti-Red Fluorescent Protein (RFP) antibody primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe) detection with ECL Western Blot Detection Kit
anti-Red Fluorescent Protein (RFP) antibody (2) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of approximately 72 kDa (predicted molecular weight of RFP-PCNA: 72 kDa). Primary antibody: 0.2 µg of RFP antibody 5F8 incubation for 1 h at RT, secondary antibody: anti-rat-Alexa647
anti-Red Fluorescent Protein (RFP) antibody (3) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
anti-Red Fluorescent Protein (RFP) antibody (4) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation for 1 h at RT. A 50 kDa band is precipitated which is IgG heavy chain whilst the 30 kDa band is PCNA.
anti-Red Fluorescent Protein (RFP) antibody (5) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primary antibody: 1/1000 RFP antibody 5F8 incubation for 1 h at RT. Secondary antibody: 1/400 donkey-anti-rat-Alexa488 incubation for 1 h at RT
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