Glutathione S-Transferase (GST) antibody

Antigen: Glutathione S-Transferase (GST)

Synonyms: gst-Pi, DmGST-2, DmGST2, DmGSTS1, DmGSTS1-1, Est2, GST, GST S1, GST-2, GST2, GSTII, GSTS1, GstS1-1, gstD2, l(2)04227, l(2)k08805, n(2)06253, DmelCG8938, CG8938, GSTA2, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE TAU 1, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE TAU 5, AT103-1A, ATGSTU1, ATGSTU5, F16P2.17, F16P2_17, GLUTATHIONE S-TRANSFERASE, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE PHI 10, ATGSTF10, ATGSTF4, EARLY DEHYDRATION-INDUCED 13, ERD13, F7F1.8, F7F1_8, GSTF10, HALIANA GLUTATHIONE S-TRANSFERASE PHI 10, ARABIDOPSIS GLUTATHIONE-S-TRANSFERASE 6, ATGSTF11, ATGSTF6, GLUTATHIONE S-TRANSFERASE F11, T17B22.12, T17B22_12, ATGSTU17, EARLY-RESPONSIVE TO DEHYDRATION 9, ERD9, F14N23.26, F14N23_26, GLUTATHIONE S-TRANSFERASE 30, GLUTATHIONE S-TRANSFERASE 30B, GLUTATHIONE S-TRANSFERASE TAU 17, GST30, GST30B, ARABIDOPSIS THALIANA GLUATIONE S-TRANSFERASE F3, ATGSTF3, EARLY RESPONSIVE TO DEHYDRATION 11, ERD11, F22D16.7, F22D16_7, GLUTATHIONE S-TRANSFERASE 1, GLUTATHIONE S-TRANSFERASE 6, GST1, GSTF6

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN334703

Additional Information

Alternative name: Glutathione-S-transferase (GST)

Format: Affinity-purified

Description: GST family of enzymescomprises a long list of cytosolic, mitochondrial, and microsomalproteinsthat are 45-55 kDa (dimer form) size and are capable of multiple reactions with a multitude of substrates, both endogenousand xenobiotic. GST catalyses the conjugation of reduced glutathione meaning the sulfhydryl group, to electrophilic centers on a wide variety of substrates. This activity is useful in the detoxification of endogenous compounds such as peroxidised lipids, as well as the metabolism of xenobiotics. GST binds toxins and function as transport protein. Glutathione S-transferase is used to create the so-called 'GST gene fusion system'. The GST is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's multiple cloning sites results in expression of a fusion protein - the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion proteins offer an important biological assay for direct protein-to-protein interactions. The GST tag has the size of 220 amino acids, which, compared to other tags like the myc- or the FLAG-tag, is quite big. It is fused to the N-terminusof a protein. However, many commercially-available sources of GST-tagged plasmids include a thrombindomain for cleavage of the GST tag during protein purification. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins. olyclonal antibody to recombinant glutathione-S-transferase (GST) was raised by immunizing rabbits with affinity purified GST. The protein was expressed in bacteria and purified by glutathione-affinity chromatography. From the crude serum, antigen-specific antibodies were purified by antigen-affinity chromatography. The product is provided as antigen-affinity purified IgG, with 0.05 % sodium azide as a preservative.

Synonyms: gst-Pi, DmGST-2, DmGST2, DmGSTS1, DmGSTS1-1, Est2, GST, GST S1, GST-2, GST2, GSTII, GSTS1, GstS1-1, gstD2, l(2)04227, l(2)k08805, n(2)06253, DmelCG8938, CG8938, GSTA2, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE TAU 1, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE TAU 5, AT103-1A, ATGSTU1, ATGSTU5, F16P2.17, F16P2_17, GLUTATHIONE S-TRANSFERASE, ARABIDOPSIS THALIANA GLUTATHIONE S-TRANSFERASE PHI 10, ATGSTF10, ATGSTF4, EARLY DEHYDRATION-INDUCED 13, ERD13, F7F1.8, F7F1_8, GSTF10, HALIANA GLUTATHIONE S-TRANSFERASE PHI 10, ARABIDOPSIS GLUTATHIONE-S-TRANSFERASE 6, ATGSTF11, ATGSTF6, GLUTATHIONE S-TRANSFERASE F11, T17B22.12, T17B22_12, ATGSTU17, EARLY-RESPONSIVE TO DEHYDRATION 9, ERD9, F14N23.26, F14N23_26, GLUTATHIONE S-TRANSFERASE 30, GLUTATHIONE S-TRANSFERASE 30B, GLUTATHIONE S-TRANSFERASE TAU 17, GST30, GST30B, ARABIDOPSIS THALIANA GLUATIONE S-TRANSFERASE F3, ATGSTF3, EARLY RESPONSIVE TO DEHYDRATION 11, ERD11, F22D16.7, F22D16_7, GLUTATHIONE S-TRANSFERASE 1, GLUTATHIONE S-TRANSFERASE 6, GST1, GSTF6

Application Details

Application Notes: The monospecific polyclonal IgG antibody against GST recognises recombinant GST. It reacts specifically to a variety of GST fusion proteins used as targets in immunoblotting experiments. It also immunoprecipitates the target proteins efficiently. This antibody is reactive with immobilized native antigens on ELISA plates as well. Polyclonal antibody against GST may be used for the capturing of GST fusion proteins using various immunochemical assays including immunobotting, dot blotting, immunoprecipitation, ELISA and immunohistochemistry (immunofluorescence or immuno-enzymatic staining). Western Blotting :1/10,000. ELISA : 1/200,000

Concentration: 1mg/1ml

Storage: For continuous use, store at 2-8

Research Area: Enzymes, Metabolism

Restrictions: For Research Use only