Epstein-Barr Virus EBNA1 antibody

Antigen: Epstein-Barr Virus EBNA1

Clonality: Monoclonal (P1B5AT)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334739

Additional Information

Immunogen: EBNA1 antibody is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant Epstein-Barr virus nuclear antigen 1 protein.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: P1B5AT

Description: The Epstein-Barr virus (EBV), also called Human herpes virus 4 (HHV-4), is a virus of the herpes family (which includes Herpes simplex virus and Cytomegalovirus. On infecting the B-lymphocyte, the linear virus genome circularizes and the virus subsequently persists within the cell as an episome. The virus can execute several distinct programs of gene expression which can be broadly categorized as being lytic cycle or latent cycle. The lytic cycle or productive infection results in staged expression of a host of viral proteins with the ultimate objective of producing infectious virions. Formally, this phase of infection does not inevitably lead to lysis of the host cell as EBV virions are produced by budding from the infected cell. The latent cycle (lysogenic) programs are those that do not result in production of virions. A very limited, distinct set of viral proteins are produced during latent cycle infection. These include Epstein-Barr nuclear antigen (EBNA)-1, EBNA-2, EBNA-3A, EBNA-3B, EBNA-3C, EBNA-leader protein (EBNA-LP) and latent membrane proteins (LMP)-1, LMP-2A and LMP-2B and the Epstein-Barr encoded RNAs (EBERs).

Application Details

Application Notes: EBNA1 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution ranges for Western blot analysis is 1:1,000 ~ 1:2,000.

Concentration: 1mg/ml containing PBS, pH-7.4, & 0.02% thimerosol.

Purification: EBNA1 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

Storage: For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C.

Restrictions: For Research Use only