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Antibodies
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anti-Interleukin 10 (IL10) antibody
Interleukin 10 (IL10) antibody
| Antigen |
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| Synonyms |
CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451 |
| Clonality |
Monoclonal (NYRmIL-10) |
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Host
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Reactivity
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Intracellular Staining (IS)
Western Blotting (WB) (55), Enzyme Immunoassay (EIA) (45), Flow Cytometry (FACS) (31), ELISA (28), Functional Studies (Func) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Radioimmunoassay (RIA) (5), ELISA (Detection) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), Intracellular Staining (IS) (3), Dot Blot (Dot) (2), ELISPOT (1), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN334749 |
| Quantity |
500ug (1mg/ml in PBS (after reconstitution...) (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Interleukin-10
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Immunogen
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recombinant Murine IL-1
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Isotype
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IgM (Matching secondary antibodies)
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Clone
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NYRmIL-10
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Description
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IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Synonyms: B-TCGF, CSIF, TGIF, IL-10, IL10A, MGC126450, MGC126451, Cytokine synthesis inhibitory factor.
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Application Notes
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By direct ELISA, 1:5,000 dilution will yield 0.1 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
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Concentration
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1mg/ml in PBS (after reconstitution)
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Purification
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Boric Acid precipitation
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Storage
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Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
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Research Area
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Cytokines, Receptors, Inflammation
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Restrictions
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For Research Use only
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Alternatives for antigen "Interleukin 10 (IL10)", type "Antibodies"
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Hosts
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Rat (48), Rabbit (24), Mouse (23), Sheep (1)
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Reactivities
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Human (52), Mouse (Murine) (31), Cow (Bovine) (6), Rat (Rattus) (6), Horse (Equine) (1), Sheep (Ovine) (1)
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Applications
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Western Blotting (WB) (55), Enzyme Immunoassay (EIA) (45), Flow Cytometry (FACS) (31), ELISA (28), Functional Studies (Func) (22), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Radioimmunoassay (RIA) (5), ELISA (Detection) (4), Immunoprecipitation (IP) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (IHC) (3), Intracellular Staining (IS) (3), Dot Blot (Dot) (2), ELISPOT (1), Immunocytochemistry (ICC) (1), Immunofluorescence (IF) (1), Neutralization (Neut) (1)
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Conjugates
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Biotin (19), PE (2), RPE (2), FITC (1)
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Epitopes
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Biotinylated (1)
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