Macrophage/Monocyte Chemotactic & Activating Factor antibody

Antigen: Macrophage/Monocyte Chemotactic & Activating Factor

Clonality: Monoclonal (YNR-HMCAF)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334754

Additional Information

Immunogen: recombinant Human MCAF

Isotype: IgG2a  (Matching secondary antibodies)

Clone: YNR-HMCAF

Description: Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). It is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kappaB (NFkappaB). CCL2 recruits immune cells, such as monocytes, to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a molecular weight of approximately 13kDa. As with many other CC chemokines, CCL2 is located on chromosome 17 in humans. The cell surface receptors that bind CCL2 are CCR2 and CCR5. Synonyms: Small inducible cytokine A2, CCL2, Monocyte chemotactic protein 1, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, MCAF, Monocyte secretory protein JE, HC11, chemokine (C-C motif) ligand 2, MCP1, SCYA2, GDCF-2, SMC-CF, HSMCR30, MGC9434, GDCF-2 HC11.

Application Details

Application Notes: In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.

Concentration: 1mg/ml in PBS (after reconstitution)

Purification: Ion exchange.

Storage: Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C. Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Research Area: Chemokines

Restrictions: For Research Use only