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Tumor Necrosis Factor (TNF) antibody

Antigen

Tumor Necrosis Factor (TNF)
Synonyms
DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF,  ... show more
Clonality Monoclonal (NYRhTNFa-E2)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives ELISA, Western Blotting (WB), Intracellular Staining (IS)
Catalog no. ABIN334758
Quantity 500ug  (1mg/ml in PBS (after reconstitution...)  (Variants)
Price 500.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name Tumor Necrosis Factor-alpha
Immunogen recombinant Human TNF-a
Isotype IgG1  (Matching secondary antibodies)
Clone NYRhTNFa-E2
Description Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer. Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
Synonyms DIF, TNFA, TNFSF2, TNF-alpha, B12, B61, EDP1, MGC2317, Edp1, Edp-1, Tnfip1, Tnfa, RATTNF, MGC124630, zgc:55302, wu:fi06b02, TNFAIP1, TNF, tnf, DKFZp468A2328, Tnfsf1a, TNFalpha, MGC151434, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, TNF-a, tnfsf2, tnf-alpha, Cachectin

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes
Concentration 1mg/ml in PBS (after reconstitution)
Purification ion exchange
Storage Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "Tumor Necrosis Factor (TNF)", type "Antibodies"
Hosts Mouse (185), Rabbit (169), Rat (51), Goat (22), Hamster (11), Sheep (5), Chicken (4), Armenian Hamster (2)
Reactivities Human (290), Mouse (Murine) (133), Rat (Rattus) (75), Pig (Porcine) (45), Horse (Equine) (42), Dog (Canine) (37), Cow (Bovine) (31), Rabbit (22), Chicken (6), Sheep (Ovine) (5), Baboon (4), Monkey (4), Guinea Pig (2)
Applications Western Blotting (WB) (210), ELISA (176), Flow Cytometry (FACS) (160), Enzyme Immunoassay (EIA) (101), Immunofluorescence (IF) (60), Immunohistochemistry (Frozen Sections) (IHC (fro)) (50), Functional Studies (Func) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (45), Neutralization (Neut) (34), Radioimmunoassay (RIA) (33), Immunoprecipitation (IP) (31), Immunohistochemistry (IHC) (24), Blocking Antibody (Inhibition) (9), ELISPOT (8), ELISA (Capture) (4), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (3), Intracellular Staining (IS) (3), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1)
Conjugates Biotin (52), FITC (20), PE (12), Cy5.5 (5), HRP (5), RPE (5), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy7,PE (4), PE,Cy5.5 (4), APC (3), Cy3 (3), Cy5 (3), Cy7 (3), Gold (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy7 (3), RBITC (3), Alexa Flour 488 (2)