CD80 (CD80) antibody
| Antigen |
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| Synonyms |
LAB7, CD28LG, CD28LG1 |
| Clonality |
Monoclonal (MB7-1) |
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Host
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Reactivity
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Application
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 Alternatives Blocking Antibody (Inhibition), Immunohistochemistry (IHC), Functional Studies (Func)
Flow Cytometry (FACS) (188), Western Blotting (WB) (53), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (48), ELISA (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Functional Studies (Func) (15), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Blocking Antibody (Inhibition) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN334765 |
| Quantity |
500ug (1mg/ml in PBS (after reconstitution...) (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CD80
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Immunogen
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Purified mouse LPS-activated B cells
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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MB7-1
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Description
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The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells CD-80 is involved in the costimulatory signal essential for t- lymphocyte activation. t-cell proliferation and cytokine production is induced by the binding of cd28 or ctla-4 to this receptor. Synonyms: LAB7, CD28LG, CD28LG1, CD28LG1, B71.
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Application Notes
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Blocking and staining antibody. For staining, use 10 ul/106 cells. Titer for blocking T cell activation should be determined by the investigator. Available Conjugates: This antibody is only available as purified antibody.
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Concentration
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1mg/ml in PBS (after reconstitution)
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Purification
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Ion exchange column
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Storage
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Storage: Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
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Research Area
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Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "CD80 (CD80)", type "Antibodies"
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Hosts
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Mouse (111), Rat (60), Rabbit (53), Hamster (18), Goat (2)
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Reactivities
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Human (115), Mouse (Murine) (109), Rat (Rattus) (29), Chicken (6), Dog (Canine) (4), Rabbit (3), Baboon (2), Cow (Bovine) (2), Horse (Equine) (2), Pig (Porcine) (1)
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Applications
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Flow Cytometry (FACS) (188), Western Blotting (WB) (53), Immunofluorescence (IF) (49), Immunoprecipitation (IP) (48), ELISA (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (20), Functional Studies (Func) (15), Enzyme Immunoassay (EIA) (11), Immunohistochemistry (IHC) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunocytochemistry (ICC) (3), Blocking Antibody (Inhibition) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Neutralization (Neut) (1)
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Conjugates
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FITC (27), PE (27), Biotin (26), APC (8), Cy5,PE (6), PE,Cy5 (4), Alexa Fluor 488 (3), RPE (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1), Pacific Blue (1), PerCP (1), RPE,Cy5 (1)
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Epitopes
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AA 45-63 (1), extra-cellular domain (1)
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