B220 (CD45R) antibody

Antigen: B220 (CD45R)

Clonality: Monoclonal (MB220)

Host: Rat

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN334767

Additional Information

Immunogen: Purified mouse LN B cells

Isotype: IgM  (Matching secondary antibodies)

Clone: MB220

Description: B220 (CD45R) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. B220 (CD45R) is required for t-cell activation through the antigen receptor. The first ptpase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Synonyms: LCA, PTPRC, LY5, B220, CD45R, T200, GP180, PROTEIN-TYROSINE PHOSPHATASE, RECEPTOR-TYPE C.

Application Details

Application Notes: Staining antibody. For staining, use 10 ul/106 cells. Available Conjugates: This antibody is only available as purified antibody.

Concentration: 1mg/ml in PBS (after reconstitution)

Purification: Ion exchange column

Storage: Storage: Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.

Restrictions: For Research Use only

Publications

Publications: Mikkelsen, Long, Zhang et al.: "Partial regulatory T cell depletion prior to acute feline immunodeficiency virus infection does not alter disease pathogenesis." in: PLoS ONE, Vol. 6, Issue 2, pp. e17183, 2011 (PubMed). Method employed by authors: Flow Cytometry (FACS) (Sample species: Cat (Feline)).