Hepatitis B Virus (AD & AY Antigens) antibody

Antigen: Hepatitis B Virus (AD & AY Antigens)

Clonality: Monoclonal (NYRHepB)

Host: Mouse

Application: ELISA

Catalog no.: ABIN334778

Additional Information

Immunogen: recombinant Hep B vaccine (BTG)

Isotype: IgG1

Clone: NYRHepB

Description: Hepatitis B is one of a few known non-retroviral viruses which employ reverse transcription as a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.

Application Details

Application Notes: By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). Additional Information: This antibody recognizes both the AD and the AY antigens in direct ELISA.

Concentration: 1mg/ml in PBS (after reconstitution)

Purification: Ion exchange column

Storage: Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.

Restrictions: For Research Use only