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CD4 Molecule (CD4) antibody

Antigen

CD4 Molecule (CD4)
Synonyms CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25
Clonality Monoclonal (HCD4)
Host
Reactivity
Alternatives

Mouse (Murine)
Application
Alternatives Blocking Antibody (Inhibition), Immunohistochemistry (IHC), Functional Studies (Func)
Catalog no. ABIN334784
Quantity 500ug  (1mg/ml in PBS (after reconstitution...)  (Variants)
Price 500.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD4
Immunogen Purified human PBL CD4+ T cells
Isotype IgG2b  (Matching secondary antibodies)
Clone HCD4
Description CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cell with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized. Synonyms: gp55, HLA-2, L3 / T4, Ly-4, T cell antigen T4/LEU3, T4, sCD4, CD4mut.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Blocking and staining antibody. Blocks blinding of HIV to CD4. For staining, use 10 ml/106 cells. Titer for blocking T cell activation should be determined by the investigator. Available Conjugates: This antibody is also available conjugated to biotin.
Concentration 1mg/ml in PBS (after reconstitution)
Purification Ion exchange column
Storage Storage: Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD4 Molecule (CD4)", type "Antibodies"
Hosts Mouse (445), Rat (207), Rabbit (68)
Reactivities Human (321), Mouse (Murine) (238), Rat (Rattus) (106), Dog (Canine) (44), Chicken (34), Cow (Bovine) (34), Sheep (Ovine) (24), Pig (Porcine) (22), Cat (Feline) (20), Rabbit (20), Bird (Avian) (8), Guinea Pig (8), Horse (Equine) (4), Duck (3), Monkey (3)
Applications Flow Cytometry (FACS) (602), Immunohistochemistry (Frozen Sections) (IHC (fro)) (149), Immunoprecipitation (IP) (93), Western Blotting (WB) (83), Immunofluorescence (IF) (53), ELISA (50), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (34), Immunohistochemistry (IHC) (28), Immunocytochemistry (ICC) (16), Cytotoxicity Test (CyTox) (11), Blocking Antibody (Inhibition) (10), Enzyme Immunoassay (EIA) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunoelectron Microscopy (IEM) (2), Blocking Antibody (Inhibition) (1), ELISA (Detection) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Neutralization (Neut) (1)
Conjugates FITC (116), PE (77), Biotin (71), APC (40), RPE (22), Cy5 (9), Cy5,PE (9), Cy5.5 (6), Cy7,PE (6), Alexa Fluor 488 (5), PE,Cy7 (5), HRP (4), PE,Cy5.5 (4), Pacific Blue (4), APC,Cy7 (3), PE,Cy5 (3), PerCP (3), SPRD (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alexa Fluor 700 (2), Cy3 (2), Cy7 (2), Gold (2), PE,Cy3 (2), RBITC (2), DY647 (1), Dyomics 647 (1), PE,DY590 (1), PE,DY647 (1), PE,Texas Red (TR) (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP,Cy5.5 (1), RPE,Cy5 (1), RPE,Cy7 (1)
Epitopes domain 1 (10), domain 2 (5), non-polymorphic (5)