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Antibodies
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anti-Epidermal Growth Factor (EGF) antibody
Epidermal Growth Factor (EGF) antibody
| Antigen |
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| Synonyms |
EGF, CEGF, LOC100218747, URG, HOMG4, AI790464 |
| Clonality |
Monoclonal (NYRhEGF) |
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Host
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Reactivity
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Application
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ELISA (87), Western Blotting (WB) (82), Immunofluorescence (IF) (57), Flow Cytometry (FACS) (54), Enzyme Immunoassay (EIA) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Neutralization (Neut) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunoprecipitation (IP) (8), Functional Studies (Func) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (5), Blocking Antibody (Inhibition) (4), Immunocytochemistry (ICC) (4), Radioimmunoassay (RIA) (4), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), ELISA (Detection) (2), Blocking Antibody (Inhibition) (1)
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| Catalog no. |
ABIN334787 |
| Quantity |
500ug (1mg/ml in PBS (after reconstitution...) (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Epidermal Growth Factor
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Immunogen
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recombinant HumanEGF
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Isotype
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IgG2b (Matching secondary antibodies)
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Clone
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NYRhEGF
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Description
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Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Synonyms: Urogastrone, URG, EGF.
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Application Notes
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By direct ELISA, 1:10,000 dilution will yield 0.3 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
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Concentration
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1mg/ml in PBS (after reconstitution)
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Purification
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Ion exchange
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Storage
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Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
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Restrictions
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For Research Use only
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Alternatives for antigen "Epidermal Growth Factor (EGF)", type "Antibodies"
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Hosts
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Rabbit (106), Mouse (59), Goat (16), Rat (3), Sheep (2), Chicken (1)
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Reactivities
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Human (109), Mouse (Murine) (49), Rat (Rattus) (29), Rabbit (5), Cow (Bovine) (4), Horse (Equine) (4), Chicken (2), Dog (Canine) (2), Pig (Porcine) (1), Sponges (Suberites domuncula) (1)
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Applications
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ELISA (87), Western Blotting (WB) (82), Immunofluorescence (IF) (57), Flow Cytometry (FACS) (54), Enzyme Immunoassay (EIA) (29), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Neutralization (Neut) (14), Immunohistochemistry (Frozen Sections) (IHC (fro)) (9), Immunoprecipitation (IP) (8), Functional Studies (Func) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (IHC) (5), Blocking Antibody (Inhibition) (4), Immunocytochemistry (ICC) (4), Radioimmunoassay (RIA) (4), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), ELISA (Detection) (2), Blocking Antibody (Inhibition) (1)
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Conjugates
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Biotin (19), HRP (6), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
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Alternatives 
