Leptin (LEP) antibody
| Antigen |
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| Synonyms |
OB, OBS, FLJ94114, ob, obese |
| Clonality |
Monoclonal (YD-HLEP4B6) |
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Host
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Reactivity
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Application
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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| Catalog no. |
ABIN334790 |
| Quantity |
500ug (1mg/ml in PBS (after reconstitution...) (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Leptin
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Immunogen
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recombinant Human Leptin
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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YD-HLEP4B6
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Description
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A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores. Synonyms: OB Protein, Obesity Protein, OBS, Obesity factor.
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Application Notes
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By direct ELISA, 1:10,000 dilution will yield 0.8 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
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Concentration
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1mg/ml in PBS (after reconstitution)
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Purification
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Ion exchange
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Storage
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Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
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Research Area
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Cardiovascular, Atherosclerosis, Hormones
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Restrictions
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For Research Use only
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Alternatives for antigen "Leptin (LEP)", type "Antibodies"
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Hosts
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Rabbit (132), Mouse (35), Goat (6), Sheep (2)
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Reactivities
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Human (103), Mouse (Murine) (93), Rat (Rattus) (79), Pig (Porcine) (35), Cow (Bovine) (19), Dog (Canine) (18), Sheep (Ovine) (17), Horse (Equine) (15), Rabbit (2), Chicken (1)
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Applications
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Western Blotting (WB) (95), Enzyme Immunoassay (EIA) (61), Immunofluorescence (IF) (61), Flow Cytometry (FACS) (55), ELISA (29), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Radioimmunoassay (RIA) (15), Immunoprecipitation (IP) (14), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Transfected cell culture (TCC) (2), Blocking Antibody (Inhibition) (1), Immunocytochemistry (ICC) (1), Immunohistochemistry (IHC) (1), Neutralization (Neut) (1)
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Conjugates
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Biotin (15), Alexa Flour 488 (3), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), RBITC (3)
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Epitopes
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C-Term (3), 92-145 cyclized (2), AA 1-24 (2), amino terminal residues 1-24 (2), mouse (1), rat (1), residues 131-145 (1)
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Alternatives 
