Factor IX antibody

Antigen: Factor IX

Synonyms: F9

Clonality: Monoclonal (NYRhFIX)

Host: Mouse

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN334817

Additional Information

Immunogen: Human Factor-IX

Isotype: IgG  (Matching secondary antibodies)

Clone: NYRhFIX

Description: Factor IX is a glycoprotein, which is synthesized in the liver. The domain structure of factor IX is similar to that of the other vitamin K dependent coagulation factors. The NH2-terminal region contains 12 g-carboxyglutamic acid (gla) residues, which facilitate the calcium dependent binding of factor IX to negatively charged phospholipid surfaces. Two domains which are homologous to epidermal growth factor (EGF) span the region between the NH2-terminal gla domain and the activation peptide (Ala-146 to Arg-180). Factor IX is activated by either factor XIa or the factor VIIa/tissue factor/phospholipid complex. Cleavage at site A yields the intermediate IXa, which is subsequently converted to the fully active form IXab by cleavage at site B. The NH2-terminal light chain (GLA and EGF domains) remains covalently attached to the COOH-terminal heavy chain by a disulfide bond. The serine protease catalytic triad (Ser-365, His 221, Asp-269) is located in the heavy chain. Factor IXab is the catalytic component of the intrinsic factor Xase complex (factor VIIIa/IXa/Ca2+/phospholipid) which proteolytically activates factor X to factor Xa.

Application Details

Application Notes: By direct ELISA, 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). For W.B. use 1ug/ml of antibody.

Purification: Protein A

Storage: Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with H20 to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.

Research Area: Coagulation

Restrictions: For Research Use only