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CD14 Molecule (CD14) antibody

Antigen

CD14 Molecule (CD14)
Synonyms CD14
Clonality Monoclonal (HCD4)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Blocking Antibody (Inhibition), Immunohistochemistry (IHC)
Catalog no. ABIN334823
Quantity 500ug  (Variants)
Price 500.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies Variants back to top

Alternative name CD14
Immunogen Purified human B Cells
Isotype IgG1  (Matching secondary antibodies)
Clone HCD4
Description CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells. Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein.

Application Details
Alternatives Secondary Antibodies Variants back to top

Application Notes Blocking and staining antibody. For staining, use 10ul/106 cells. Titer for blocking LPS binding should be determined by the investigator. Available Conjugates: This antibody is also available conjugated to biotin and FITC. For staining with biotin or FITC-conjugated antibody use 5-10ul/106 cells.
Purification Ion exchange column
Storage Storage: Lyophilized: store at 4(C. After reconstitution, if not intended for use within a month, aliquot and store at -20(C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4(C.
Research Area Stem Cells, Innate Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells, Apoptosis/Necrosis, Immunology, Inflammation
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies Variants back to top

Alternatives for antigen "CD14 Molecule (CD14)", type "Antibodies"
Hosts Mouse (240), Rabbit (36), Goat (8), Rat (2), Chicken (1)
Reactivities Human (265), Pig (Porcine) (34), Mouse (Murine) (30), Cow (Bovine) (28), Dog (Canine) (26), Sheep (Ovine) (25), Rabbit (24), Monkey (23), Primates (19), Rat (Rattus) (18), Goat (9), Cat (Feline) (8), Primate (8), Chicken (1), Llama (1)
Applications Flow Cytometry (FACS) (218), Western Blotting (WB) (55), ELISA (37), Immunoprecipitation (IP) (32), Functional Studies (Func) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (22), Immunofluorescence (IF) (20), Enzyme Immunoassay (EIA) (19), Immunohistochemistry (IHC) (15), ELISA (Detection) (5), Immunocytochemistry (ICC) (3), Blocking Antibody (Inhibition) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
Conjugates FITC (35), PE (24), Biotin (21), APC (16), RPE (8), Cy5.5 (5), Alexa Fluor 488 (3), Pacific Blue (3), Alexa Fluor 700 (2), Cy5 (2), Cy7,PE (2), PE,Cy5 (2), PE,Cy7 (2), PE,DY590 (2), PE,DY647 (2), SPRD (2), APC,Cy7 (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), CF (1), Cy3 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), PE-Dyomics 590 (1), PE-Dyomics 647 (1), PerCP (1), RBITC (1), RPE,Cy5 (1), RPE,Cy5.5 (1)
Epitopes C-Term (2), N-Term (2)