MHC Class II I-A antibody
| Antigen |
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| Clonality |
Monoclonal (NYRmI-A) |
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Host
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Application
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 Alternatives Immunohistochemistry (IHC), Cytotoxicity Test (CyTox)
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| Catalog no. |
ABIN334827 |
| Quantity |
500ug (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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MHC Class II (I-A)
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Immunogen
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Purified mouse LN B cells (C3H anti-C57Bl6)
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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NYRmI-A
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Description
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MHC Class II is consists of 2 membrane spanning proteins. Each of these proteins is roughly 30 kDa, and has of two Globular domains, Alpha-1, Alpha-2, Beta-1 and Beta-2. The two regions farthest away from the membrane are alpha-1 and beta-1. The two proteins link without Covalent Bonds. The MHC Class II protein mainly presents peptides, which have been digested from external sources. MHC Class II is normally only expressed by Antigen Presenting Cells (APC) which digest foreign protein. Within the RER the alpha and beta proteins of the molecule, associate with each other, while a third protein called the invariant chain, is necessary for stabilizing the complex, without it, they will not associate. The MHC-invariant complex is then passed from the RER, into and out of, the Golgi body. It fuses with an Endocytic compartment, where an external protein has been sampled and degraded.
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Application Notes
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Cytotoxic and staining antibody. For staining, use 10 ml/106 cells. Titer for cytotoxicity should be determined by the investigator. Available Conjugates: This antibody is also available conjugated to biotin and FITC.
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Purification
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Ion exchange column
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Storage
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Storage: Lyophilized: store at 4(C. After reconstitution, if not intended for use within a month, aliquot and store at -20(C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4(C.
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Restrictions
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For Research Use only
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Alternatives for antigen "MHC Class II I-A", type "Antibodies"
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Hosts
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Mouse (22), Rat (6)
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Reactivities
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Mouse (Murine) (8), Rat (Rattus) (7), Chicken (4)
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Applications
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Flow Cytometry (FACS) (17), Cytotoxicity Test (CyTox) (12), Immunohistochemistry (IHC) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Western Blotting (WB) (2), Functional Studies (Func) (1), Immunoprecipitation (IP) (1)
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Conjugates
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FITC (7), Biotin (6), PE (3), Cy5,PE (1)
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