Interleukin 2 (IL2) antibody
| Antigen |
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| Synonyms |
IL-2, TCGF, lymphokine, Il-2, IL2, POIL2, il2 |
| Clonality |
Monoclonal (YNRhIL2) |
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Host
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Reactivity
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Human (68), Mouse (Murine) (39), Rat (Rattus) (11), Pig (Porcine) (6), Cow (Bovine) (4), Monkey (4), Sheep (Ovine) (4), Chicken (2), Bird (Avian) (1), Cat (Feline) (1), Mammalian (1)
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Functional Studies (Func)
Enzyme Immunoassay (EIA) (70), Western Blotting (WB) (51), ELISA (34), Flow Cytometry (FACS) (22), Functional Studies (Func) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (8), Dot Blot (Dot) (7), Blocking Antibody (Inhibition) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunofluorescence (IF) (4), Radioimmunoassay (RIA) (4), Neutralization (Neut) (3), ELISA (Capture) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN334834 |
| Quantity |
500ug (1mg/ml in PBS (after reconstitution...) (Variants) |
| Price |
500.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Interleukin-2
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Immunogen
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r.Human IL-2
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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YNRhIL2
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Description
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IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
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Application Notes
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By direct ELISA: 1:10,000 dilution will yield 0.4 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). 1:50 dilution will block 70-80% of CTLL proliferative response to 50 units of r.human IL-2. A certain degree of functional cross reactivity with murine IL-2 was reported by some investigators.
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Concentration
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1mg/ml in PBS (after reconstitution)
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Purification
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Ion exchange
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Storage
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In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4°C. Antibody is shipped lyophilized at ambient temperature.Shelf Life: Two years lyophilized, one month in solution at 4°C.
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Research Area
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Cytokines, Receptors, Inflammation
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Restrictions
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For Research Use only
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Alternatives for antigen "Interleukin 2 (IL2)", type "Antibodies"
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Hosts
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Mouse (62), Rat (37), Rabbit (34), Goat (5), Chicken (1), Sheep (1)
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Reactivities
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Human (68), Mouse (Murine) (39), Rat (Rattus) (11), Pig (Porcine) (6), Cow (Bovine) (4), Monkey (4), Sheep (Ovine) (4), Chicken (2), Bird (Avian) (1), Cat (Feline) (1), Mammalian (1)
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Applications
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Enzyme Immunoassay (EIA) (70), Western Blotting (WB) (51), ELISA (34), Flow Cytometry (FACS) (22), Functional Studies (Func) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (8), Dot Blot (Dot) (7), Blocking Antibody (Inhibition) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunofluorescence (IF) (4), Radioimmunoassay (RIA) (4), Neutralization (Neut) (3), ELISA (Capture) (2), Immunohistochemistry (IHC) (2), ELISA (Detection) (1)
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Conjugates
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Biotin (14), FITC (5), PE (2), RPE (2), HRP (1)
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Epitopes
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Biotinylated (3), Center (1)
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