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CD3 (CD3E) antibody (Biotin)

Antigen

CD3 (CD3E)
Synonyms T3E, TCRE, FLJ18683
Clonality Monoclonal (HCD3)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Conjugate
Alternatives Biotin
Application
Alternatives Blocking Antibody (Inhibition), Immunohistochemistry (IHC), Functional Studies (Func)
Catalog no. ABIN334850
Quantity 500ug  (1mg/ml in PBS (after reconstitution...)  (Variants)
Price 630.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Variants back to top

Alternative name CD3
Immunogen Purified human PBL T cells
Isotype IgG2a
Clone HCD3
Description CD3 has four peptide chains (gamma, delta, epsilon and zeta) that form CD3. Defects in CD3 are associated with T cell immunodeficiency CD3 complex mediates signal transduction.

Application Details
Alternatives Variants back to top

Application Notes Blocking, staining and activating antibody. For staining, use 10ul/106 cells. Titer for blocking and activating T cells should be determined by the investigator. Available Conjugates: This antibody is also available conjugated to FITC.
Concentration 1mg/ml in PBS (after reconstitution)
Purification Ion exchange column
Storage Storage: Lyophilized: store at 4°C. After reconstitution, if not intended for use within a month, aliquot and store at -20°C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4°C.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Alternatives
Application Details Variants back to top

Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
Hosts Mouse (309), Rat (55), Rabbit (51), Hamster (38)
Reactivities Human (292), Mouse (Murine) (105), Rat (Rattus) (43), Dog (Canine) (30), Pig (Porcine) (26), Chicken (18), Cow (Bovine) (17), Monkey (15), Primate (9), Bird (Avian) (4), Cat (Feline) (4), Hamster (3), Horse (Equine) (3), Primates (3), Rhesus Monkey (2), Rabbit (1)
Applications Flow Cytometry (FACS) (354), Immunohistochemistry (Frozen Sections) (IHC (fro)) (92), Immunoprecipitation (IP) (66), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (49), Functional Studies (Func) (48), Western Blotting (WB) (42), Immunofluorescence (IF) (32), ELISA (19), Immunohistochemistry (IHC) (16), Blocking Antibody (Inhibition) (9), Enzyme Immunoassay (EIA) (7), Immunocytochemistry (ICC) (6), Cytotoxicity Test (CyTox) (2), Dot Blot (Dot) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Radioimmunoassay (RIA) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates FITC (76), Biotin (45), PE (39), APC (24), RPE (10), SPRD (7), Cy5 (6), Alexa Fluor 488 (4), Cy5,PE (4), DY647 (3), PE,Cy5 (3), PE,Cy7 (3), APC,Cy7 (2), Alexa Fluor 647 (2), Cy7,PE (2), PE,Cy5.5 (2), PE,DY647 (2), PE-Dyomics 647 (2), PerCP (2), RPE,Cy5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), Pacific Blue (1), RBITC (1), RPE,Cy7 (1)
Epitopes AA 156-168 (4), AA 196-206 (1), C-Term (1)