MHC Class I (H-2K) antibody (Biotin)

Antigen: MHC Class I (H-2K)

Clonality: Monoclonal (NYRmH-2K)

Host: Mouse

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Cytotoxicity Test (CyTox)

Catalog no.: ABIN334866

Additional Information

Immunogen: Purified mouse LN cells (C57Bl/6 anti-BALB/c)

Isotype: IgG2b

Clone: NYRmH-2K

Description: MHC Class I is a membrane spanning molecule consisting of 2 proteins. MHC Class I is just about 350 amino acids in length, with roughly 75 amino acids at the carboxylic end comprising the transmembrane and cytoplasmic portions. The remaining 270 amino acids, are divided into three globular domains labeled Alpha-1, Alpha-2 and Alpha-3 prime, with Alpha-1 being closest to the amino terminus and Alpha-3 closest to the membrane. The second portion of the molecule is a small globular protein called Beta-2 Microglobulin It links primarily with the Alpha-3 prime domain and is essential for MHC stability. The MHC ability to show a vast range of antigenic peptides for T cell recognition needs a compromise between broad specificity and high affinity. Both MHC class I and MHC class II show an exceptional structure while the peptide main chain is tightly bound and peptide side chains show less restrictive interactions. It is primarily the peptide side-chain contacts and conformational variability that ensures that the peptide-MHC complex presents an antigenically unique surface to T cell receptors.

Application Details

Application Notes: Cytotoxic and staining antibody. For staining, use 10 ml/106 cells. Titer for cytotoxicity should be determined by the investigator. Available Conjugates: This antibody is also available as unconjugated antibody and conjugated to FITC.

Purification: Ion exchange column

Storage: Storage: Lyophilized: store at 4(C. After reconstitution, if not intended for use within a month, aliquot and store at -20(C. Reconstitution: Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4(C.

Restrictions: For Research Use only