Heparanase (HPSE) antibody

Antigen: Heparanase (HPSE)

Synonyms: HPA, HPR1, HSE1, HPSE1, Hpa, Hpr1, Hep, HPSE, im:7144134

Clonality: Monoclonal (HP3-17)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN334981

Additional Information

Alternative name: Heparanase-1

Immunogen: Mab HP3/17 is a Protein G affinity purified monoclonal antibody raised against a polypeptide from the 50 kDa subunit of Heparanase.

Isotype: IgG2b kappa  (Matching secondary antibodies)

Clone: HP3-17

Description: Heparanase is an endo b-D-glucuronidase, which degrades heparin sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).

Application Details

Application Notes: HP3/17 reacts with the 50 kDa subunit and with the 65 kDa precursor of human or mouse Heparanase by Western blotting and immunohistochemistry. Recommended dilution range for Western blot analysis: 1:4000. Recommended dilution range for immunohistochemistry: 1:40.

Purity: >98% on SDS-PAGE when loaded 50 ug/lane.

Buffer: Each vial contains 50, 100 or 150 ug in 12.5, 25 or 37.5 ul respectively, of 0.22 micron filtered solution of 20 mM Sodium Phosphate 150 mM NaCl

Storage: Store at 4 degree C. Stable for six months from the date of shipment. For extended storage, freeze in working aliquots at -20 degree C. Avoid repeated freeze-thaw cycles.

Restrictions: For Research Use only