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Antibodies
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anti-Vimentin (VIM) antibody (Biotin)
Vimentin (VIM) antibody (Biotin)
| Antigen |
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| Synonyms |
FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime |
| Clonality |
Monoclonal (HVimentin) |
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Host
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Reactivity
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Human (156), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (32), Cow (Bovine) (28), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Conjugate
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FITC (10), Biotin (7), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Application
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Western Blotting (WB) (106), Immunofluorescence (IF) (74), Flow Cytometry (FACS) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Immunohistochemistry (IHC) (54), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (16), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
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| Catalog no. |
ABIN334991 |
| Quantity |
500ug (Variants) |
| Price |
770.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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Vimentin
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Immunogen
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Purified Human Vimentin
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Isotype
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IgG1
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Clone
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HVimentin
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Description
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Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines.
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Application Notes
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For intra-cellular staining, use 5-10ul/106 cells. Wash twice with PBS/Azide before adding the appropriate amount of secondary fluorescent reagent. Cross Reactivity: Vimentin antibody will recognize most mammalian Vimentin except mouse and rat.
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Purification
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Ion exchange column
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Storage
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Shipping Condition: Antibody is shipped lyophilized at ambient temperature. Storage: In lyophilized form, for long periods, store at 4(C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20(C. Reconstitution: Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf Life: Two years lyophilized, one month in solution at 4(C.
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Research Area
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Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
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Restrictions
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For Research Use only
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Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
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Hosts
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Rabbit (95), Mouse (73), Chicken (9), Sheep (3), Guinea Pig (2), Goat (1)
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Reactivities
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Human (156), Rat (Rattus) (66), Mouse (Murine) (60), Chicken (32), Cow (Bovine) (28), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (6), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (106), Immunofluorescence (IF) (74), Flow Cytometry (FACS) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Immunohistochemistry (IHC) (54), ELISA (39), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (16), Immunoprecipitation (IP) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
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Conjugates
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FITC (10), Biotin (7), Alexa Fluor 488 (4), PE (4), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Epitopes
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pSer82 (21), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)
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Alternatives 
