SH2D1B antibody (Internal Region)

Catalog No. ABIN335133

Product Details anti-SH2D1B Antibody

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SH2D1B antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purpose: EAT2 phospho (Y127)

Sequence: NSNSDpYVDV LP

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Phosphorylated peptide with sequence C-NSNSDpYVDVLP, from the internal region of the protein sequence according to NP_444512.2

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Approx 14 kDa band observed in lysates of cell line A431 (calculated MW of 16.6 kDa according to NP_002643.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-SH2D1B antibody (ABIN335133)

Clarkson, Simmonds, Puklavec, Brown: "Direct and indirect interactions of the cytoplasmic region of CD244 (2B4) in mice and humans with FYN kinase." in: The Journal of biological chemistry, Vol. 282, Issue 35, pp. 25385-94, (2007) (PubMed).

Target Details for SH2D1B

Target: SH2D1B (SH2 Domain Containing 1B (SH2D1B))

Alternative Name: SH2D1B (SH2D1B Products)

Synonyms: EAT2 antibody, Sh2d1b1 antibody, RGD1563935 antibody, SH2 domain containing 1B antibody, SH2D1B antibody, Sh2d1b antibody

Background: SH2D1B, SH2 domain containing 1B, EAT2, SH2 domain-containing molecule EAT2

Gene ID: 117157

NCBI Accession: NP_444512

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process