Keratin 8 (KRT8) antibody

Antigen: Keratin 8 (KRT8)

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC76893, wu:fa91a11, wu:fd19e09, wu:fl23c09, etID20321.6, zfk8, DreK8, cb186, krt2-8, MGC85764, sb:cb186, MGC174782, wu:fa20h05, wu:fa95h10, wu:fb96c06, DKFZp468F2127

Clonality: Monoclonal (M20)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335211

Additional Information

Alternative name: Cytokeratin 8 / Keratin K8

Immunogen: M20 is a mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a mouse immunized with keratin isolated from the human breast carcinoma cell line MCF-7.

Format: Culture supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: M20

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human and mouse.

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC76893, wu:fa91a11, wu:fd19e09, wu:fl23c09, etID20321.6, zfk8, DreK8, cb186, krt2-8, MGC85764, sb:cb186, MGC174782, wu:fa20h05, wu:fa95h10, wu:fb96c06, DKFZp468F2127

Application Details

Application Notes: M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1: 5 - 1:10 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:5 - 1: 20 for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Keratin 8 (KRT8) antibody

anti-Keratin 8 (KRT8) antibody (Image 2)

anti-Keratin 8 (KRT8) antibody (Image 3)

Publications

Publications: Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ivanyi, Groeneveld, Van Doornewaard et al.: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).

Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." in: Histochemistry, Vol. 91, Issue 2, pp. 151-9, 1989 (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).