Keratin 10 (KRT10) antibody

Antigen: Keratin 10 (KRT10)

Synonyms: K10, KPP, CK10, K1C1, Krt1-10, Krt-1.10, D130054E02Rik, KRT10, KRT9, Ka10, Ker10

Clonality: Monoclonal (RKSE60)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335212

Additional Information

Alternative name: Cytokeratin 10 / Keratin K10

Immunogen: RKSE60 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with cytokeratins from the human epidermis.

Format: Culture supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: RKSE60

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human, mouse, rat, canine.

Application Details

Application Notes: RKSE60 reacts exclusively with cytokeratin 10 which is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas. RKSE60 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and flow cytometry. Optimal antibody dilution should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Keratin 10 (KRT10) antibody

anti-Keratin 10 (KRT10) antibody (Image 2)

anti-Keratin 10 (KRT10) antibody (Image 3)

Publications

Publications: Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Puts, Moesker, Kenemans et al.: "Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix." in: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists, Vol. 4, Issue 4, pp. 300-13, 1986 (PubMed).

Broers, Carney, Klein Rot et al.: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, pp. 37-60, 1987 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).

Ramaekers, Puts, Moesker et al.: "Antibodies to intermediate filament proteins in the immunohistochemical identification of human tumours: an overview." in: The Histochemical journal, Vol. 15, Issue 7, pp. 691-713, 1983 (PubMed).

Meek, Van Elssen, Huijskens et al.: "T cells fail to develop in the human skin-cell explants system; an inconvenient truth." in: BMC immunology, Vol. 12, Issue 1, pp. 17, 2011 (PubMed).