Cytokeratin 10+13 / Keratin K10+K13 antibody

Antigen: Cytokeratin 10+13 / Keratin K10+K13

Clonality: Monoclonal (DE-K13)

Host: Mouse

Reactivity: Human, Cat (Feline)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335214

Additional Information

Immunogen: DE-K13 is a mouse monoclonal IgG2a, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 mouse immunized with a cytoskeletal preparation extracted from human ectocervical epithelium.

Format: Culture supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: DE-K13

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human and feline.

Application Details

Application Notes: DE-K13 reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues. DE-K13 is useful for immunoblotting, immunocytochemistry, flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues (see also above) and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. For staining on paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room temperature is required. Optimal antibody dilution should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Cytokeratin 10+13 / Keratin K10+K13 antibody

Publications

Publications: Ivanyi, Minke, Hageman et al.: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992 (PubMed).

van Bommel, Kenemans, Helmerhorst et al.: "Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix." in: Cancer, Vol. 74, Issue 8, pp. 2314-20, 1994 (PubMed).

Ivanyi, Minke, Hageman et al.: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, 1993 (PubMed).