Cytokeratin 13 (KRT13) antibody

Antigen: Cytokeratin 13 (KRT13)

Synonyms: K13, CK13, MGC3781, MGC161462, Krt1-13, Krt-1.13

Clonality: Monoclonal (1C7)

Host: Mouse

Reactivity: Human, Zebrafish (Danio rerio)

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335215

Additional Information

Alternative name: Cytokeratin 13 / Keratin K13

Immunogen: 1C7 is a mouse monoclonal IgG2a antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytokeratin preparation extracted from human esophagus.

Isotype: IgG2a

Clone: 1C7

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: Human.

Application Details

Application Notes: 1C7 reacts exclusively with cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia. 1C7 is suitable for immunoblotting and immunohistochemistry on frozen tissues and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration. .

Purification: Culture supernatant

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Bauwens, De Groot, Ramaekers et al.: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky et al.: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

van Niekerk, Boerman, Ramaekers et al.: "Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas." in: The American journal of pathology, Vol. 138, Issue 2, pp. 455-63, 1991 (PubMed).

van Muijen, Ruiter, Franke et al.: "Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13." in: Experimental cell research, Vol. 162, Issue 1, pp. 97-113, 1986 (PubMed).

Weikel, Wagner, Moll: "Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in reserve cells." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 54, Issue 2, pp. 98-110, 1988 (PubMed).

Van Niekerk, Ramaekers, Hanselaar et al.: "Changes in expression of differentiation markers between normal ovarian cells and derived tumors." in: The American journal of pathology, Vol. 142, Issue 1, pp. 157-77, 1993 (PubMed).