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Antibody
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anti-Keratin 14 KRT14
Keratin 14 (KRT14) antibody
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| Synonyms |
K14, NFJ, CK14, EBS3, EBS4, Krt1-14, AI626930, Krt-1.14, Krt14l, Krt14, Ka14, KRT14, ouro2 |
| Clonality |
Monoclonal (SPK-14) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Western Blotting (WB) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunohistochemistry (IHC) (12), Flow Cytometry (FACS) (9), Immunofluorescence (IF) (6), ELISA (3), Enzyme Immunoassay (EIA) (3), ELISA (Detection) (2), Immunocytochemistry (ICC) (2), Immunoprecipitation (IP) (1)
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| Catalog no. |
ABIN335217 |
| Quantity |
1ml |
| Price |
286.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business days |
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Alternative name
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Cytokeratin 14 / Keratin K14
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Immunogen
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SPK-14 is a mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a (BALB/c x B6)F1 mouse immunized with a synthetic peptide corresponding to the carboxy-terminal sequence of human cytokeratin 14 (KVVSTHEQVLRTKN) coupled to keyhole limpet hemocyanin.
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Format
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Culture supernatant
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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SPK-14
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Description
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Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
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Specificity
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Human, feline and pig.
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Application Notes
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SPK-14 reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia. SPK-14 is useful for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration.
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Storage
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Store at 4 o C, or in small aliquots at -20 o C.
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Restrictions
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For Research Use only
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Publications
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Ivanyi, Minke, Hageman et al.: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992 (PubMed).
Bonfrer, Groeneveld, Korse et al.: "Monoclonal antibody M3 used in tissue polypeptide-specific antigen assay for the quantification of tissue polypeptide antigen recognizes keratin 18." in: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, Vol. 15, Issue 4, pp. 210-22, 1994 (PubMed).
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Alternatives for antigen "Keratin 14 (KRT14)", type "Antibodies"
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Hosts
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Rabbit (22), Mouse (21), Guinea Pig (2)
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Reactivities
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Human (41), Rat (Rattus) (7), Mouse (Murine) (5), Dog (Canine) (2), Pig (Porcine) (2), Cow (Bovine) (1)
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Applications
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Western Blotting (WB) (25), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (13), Immunohistochemistry (IHC) (12), Flow Cytometry (FACS) (9), Immunofluorescence (IF) (6), ELISA (3), Enzyme Immunoassay (EIA) (3), ELISA (Detection) (2), Immunocytochemistry (ICC) (2), Immunoprecipitation (IP) (1)
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Conjugates
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FITC (1)
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Epitopes
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C-Term (3), N-Term (2), Center (1)
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