Keratin Basal Cell antibody

Antigen: Keratin Basal Cell

Clonality: Monoclonal (RCK103)

Host: Mouse

Reactivity: Human, Rat (Rattus), Pig (Porcine), Rabbit, Hamster, Dog (Canine), Guinea Pig, Chicken, Quail

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335225

Additional Information

Alternative name: Basal cell cytokeratin

Immunogen: RCK103 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a mix of cell preparations containing human cytokeratins.

Format: Culture supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: RCK103

Description: Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

Specificity: RCK103 is a cytokeratin antibody recognizing (amongst others) cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.

Application Details

Application Notes: RCK103 is useful for immunoblotting, immunocytochemistry, immunohistochemistryand for immunohistochemistry on frozen tissues and with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and flow cytometry. Optimal antibody dilution should be determined by titration.

Storage: Store at 4°C, or in small aliquots at -20°C.

Research Area: Extracellular Matrix, Cancer, Cytokeratins

Restrictions: For Research Use only

Publications

Publications: Vos, van den Ingh, de Neijs et al.: "Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord." in: Zentralblatt für Veterinärmedizin. Reihe A, Vol. 39, Issue 10, pp. 721-40, 1993 (PubMed).

Smedts, Ramaekers, Robben et al.: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Kuijpers, Tonnaer, Peters et al.: "Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig." in: Hearing research, Vol. 52, Issue 1, pp. 133-46, 1991 (PubMed).

Verhagen, Aalders, Ramaekers et al.: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, 1988 (PubMed).

Schaafsma, Ramaekers, van Muijen et al.: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." in: Histochemistry, Vol. 91, Issue 2, pp. 151-9, 1989 (PubMed).

Feitz, Debruyne, Vooijs et al.: "Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues." in: The Journal of urology, Vol. 136, Issue 4, pp. 922-31, 1986 (PubMed).

Vos, van den Ingh, Ramaekers et al.: "The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region." in: Veterinary pathology, Vol. 30, Issue 4, pp. 352-61, 1993 (PubMed).