Desmin (DES) antibody

Antigen: Desmin (DES)

Synonyms: DES, des-a, MGC52614, des-b, MGC80853, CSM1, CSM2, CMD1I, FLJ12025, FLJ39719, FLJ41013, FLJ41793, des, csm1, csm2, desm, cmd1i, MGC75911, LOC395906, LOC100220724, desmin, cb290, fb59a12, MGC109859, wu:fb59a12, zgc:109859, MGC154009, wu:fc11d08

Clonality: Monoclonal (RD301)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Rabbit, Hamster, Dog (Canine), Chicken, Pig (Porcine)

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335226

Additional Information

Alternative name: Desmin

Immunogen: RD301 is a mouse monoclonal IgG2b antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal desmin extract of chicken gizzard.

Format: Culture supernatant

Isotype: IgG2b  (Matching secondary antibodies)

Clone: RD301

Description: Desmin is a 53 kDa intermediate filament protein that exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Specificity: Human, mouse, rat, rabbit, hamster, canine, chicken and swine.

Synonyms: DES, des-a, MGC52614, des-b, MGC80853, CSM1, CSM2, CMD1I, FLJ12025, FLJ39719, FLJ41013, FLJ41793, des, csm1, csm2, desm, cmd1i, MGC75911, LOC395906, LOC100220724, desmin, cb290, fb59a12, MGC109859, wu:fb59a12, zgc:109859, MGC154009, wu:fc11d08

Application Details

Application Notes: RD301 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. RD301 is suitable for immunoblotting and immunohistochemistry on frozen tissues with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Cancer, Cytoskeleton

Restrictions: For Research Use only

Images

anti-Desmin (DES) antibody

anti-Desmin (DES) antibody (Image 2)

Publications

Publications: Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Verhagen, Aalders, Ramaekers et al.: "Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration." in: The Prostate, Vol. 13, Issue 1, pp. 25-38, 1988 (PubMed).

Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).

Schaart, Viebahn, Langmann et al.: "Desmin and titin expression in early postimplantation mouse embryos." in: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, 1990 (PubMed).

Quax, van den Broek, Egberts et al.: "Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer." in: Cell, Vol. 43, Issue 1, pp. 327-38, 1986 (PubMed).

Dispersyn, Geuens, Ver Donck et al.: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, 2001 (PubMed).