Lamin A/C (LMNA) antibody

Antigen: Lamin A/C (LMNA)

Synonyms: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Clonality: Monoclonal (133A2)

Host: Mouse

Reactivity: Human, Rat (Rattus), Mouse (Murine), Cow (Bovine), Dog (Canine)

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335242

Additional Information

Alternative name: Lamin A

Immunogen: 133A2 is a mouse monoclonal IgG3/kappa antibody obtained from fusion of P3/X63.Ag8.653 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with partially purified recombinant human lamin A.

Format: Culture supernatant

Isotype: IgG3  (Matching secondary antibodies)

Clone: 133A2

Description: Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2. Recent evidence has revealed that mutations in A-type lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type lamins coincides with cell differentiation and as A-type lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type lamins.

Specificity: Human, rat, mouse, bovine, dog.

Synonyms: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Application Details

Application Notes: 133A2 recognizes an epitope located between residues 598-611 of lamin A and therefore 133A2 reacts exclusively with lamin A. 133A2 is suitable for immunocytochemistry, immunohistochemistry on frozen sections, immunoblotting and flow cytometry and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, . Optimal antibody dilution should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Images

anti-Lamin A/C (LMNA) antibody

Publications

Publications: Hozuak, Sasseville, Raymond et al.: "Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells." in: Journal of cell science, Vol. 108 ( Pt 2), pp. 635-44, 1995 (PubMed).

Machiels, Broers, Raymond et al.: "Abnormal A-type lamin organization in a human lung carcinoma cell line." in: European journal of cell biology, Vol. 67, Issue 4, pp. 328-35, 1996 (PubMed).

Broers, Machiels, Kuijpers et al.: "A- and B-type lamins are differentially expressed in normal human tissues." in: Histochemistry and cell biology, Vol. 107, Issue 6, pp. 505-17, 1997 (PubMed).

Machiels, Ramaekers, Kuijpers et al.: "Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults." in: The Journal of pathology, Vol. 182, Issue 2, pp. 197-204, 1997 (PubMed).

Jansen, Machiels, Hopman et al.: "Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease." in: Histopathology, Vol. 31, Issue 4, pp. 304-12, 1997 (PubMed).

Pugh, Coates, Lane et al.: "Distinct nuclear assembly pathways for lamins A and C lead to their increase during quiescence in Swiss 3T3 cells." in: Journal of cell science, Vol. 110 ( Pt 19), pp. 2483-93, 1997 (PubMed).

Neri, Raymond, Giordano et al.: "Spatial distribution of lamin A and B1 in the K562 cell nuclear matrix stabilized with metal ions." in: Journal of cellular biochemistry, Vol. 75, Issue 1, pp. 36-45, 1999 (PubMed).