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Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN335271, Supplier: Log in to see
Antigen
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken, Goat, Dog (Canine), Monkey, Zebrafish (Danio rerio)
576
165
147
73
53
41
25
24
14
11
9
8
8
5
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
310
261
30
12
11
6
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
23
21
13
10
9
5
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
477
237
185
142
123
84
84
51
47
23
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Immunogen RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Clone RV202
Isotype IgG1
Specificity Human, mouse, rat, hamster, chicken, goat, canine and monkey.
Purification Culture supernatant
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis
Application Notes RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagentand flow cytometry. Optimal antibody dilution should be determined by titration.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Supplier Images
 image for anti-Vimentin antibody (VIM) (ABIN335271) anti-Vimentin (VIM) antibody
 image for anti-Vimentin antibody (VIM) (ABIN335271) anti-Vimentin (VIM) antibody (Image 2)
Product cited in: Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987