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Vimentin (VIM) antibody

Antigen

Vimentin (VIM)
Synonyms FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Clonality Monoclonal (RV202)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus), Hamster, Chicken, Goat, Dog (Canine), Monkey, Zebrafish (Danio rerio)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
5 references available
Catalog no. ABIN335271
Quantity 1ml  (Variants)
Price 343.20 $   Plus shipping costs $45.00
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anti-Vimentin (VIM) antibody

anti-Vimentin (VIM) antibody (2)

Additional Information
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Alternative name Vimentin
Immunogen RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
Isotype IgG1
Clone RV202
Description Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
Specificity Human, mouse, rat, hamster, chicken, goat, canine and monkey.

Application Details
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Application Notes RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagentand flow cytometry. Optimal antibody dilution should be determined by titration.
Purification Culture supernatant
Storage Store at 4 o C, or in small aliquots at -20 o C.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Restrictions For Research Use only

Publications
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Ramaekers, van Niekerk, Poels et al.: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Raats, Pieper, Vree Egberts et al.: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, 1990 (PubMed).

Ramaekers, Huysmans, Schaart et al.: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, 1988 (PubMed).

Pieper, Schaart, Krimpenfort et al.: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, 1989 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
Hosts Mouse (146), Rabbit (120), Chicken (17), Sheep (6), Guinea Pig (4), Rat (4), Goat (3)
Reactivities Human (234), Rat (Rattus) (90), Mouse (Murine) (78), Cow (Bovine) (60), Pig (Porcine) (54), Chicken (41), Dog (Canine) (35), Sheep (Ovine) (24), Horse (Equine) (18), Mammalian (11), Goat (9), Monkey (8), Hamster (7), Zebrafish (6), Guinea Pig (5), Amphibian (4), Potoroo (2), Xenopus laevis (2), Cat (Feline) (1), Frog (1), Primate (1), Rabbit (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (184), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (109), ELISA (91), Immunofluorescence (IF) (89), Immunohistochemistry (IHC) (77), Immunocytochemistry (ICC) (52), Flow Cytometry (FACS) (31), Immunoprecipitation (IP) (22), Immunohistochemistry (Frozen Sections) (IHC (fro)) (17), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoelectrophoresis (IEP) (3), Intracellular Staining (IS) (3), To be determined by user (TBD) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)) (1), Transfected cell culture (TCC) (1)
Conjugates FITC (12), Biotin (11), Alexa Fluor 488 (7), PE (7), HRP (6), Dyomics 547 (4), Alexa Fluor 350 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), DY547 (2), DyLight 549 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer82 (22), pSer72 (13), pSer56 (6), C-Term (5), N-Term (5), Internal Region (3), Center (2), Ser82 (2), pSer38 (2), AA 2-466 (1), AA 300-350 (1), AA 400-500 (1), AA 70-120 (1), pSer55 (1), pSer71 (1), pSer83 (1), pTyr996 (1)