K-Cadherin/Cadherin-6 antibody

Antigen: K-Cadherin/Cadherin-6

Clonality: Monoclonal (2B6)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335291

Additional Information

Immunogen: 2B6 is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with affinity purified extracellular domain of human cadherin-6-GST fusion protein.

Format: Culture supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 2B6

Description: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. Cadherin-6 is expressed in the proximal tubule epithelium of the kidney from which most renal cell carcinomas are derived.

Specificity: Human and rat.

Application Details

Application Notes: 2B6 recognizes the extracellular domain of cadherin-6. 2B6 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . Optimal antibody dilution should be determined by titration for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and for immunoblotting applications.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-K-Cadherin/Cadherin-6 antibody

anti-K-Cadherin/Cadherin-6 antibody (Image 2)

Publications

Publications: Shimazui, Oosterwijk, Akaza et al.: "Expression of cadherin-6 as a novel diagnostic tool to predict prognosis of patients with E-cadherin-absent renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 4, Issue 10, pp. 2419-24, 1998 (PubMed).

Shimazui, Oosterwijk-Wakka, Akaza et al.: "Alterations in expression of cadherin-6 and E-cadherin during kidney development and in renal cell carcinoma." in: European urology, Vol. 38, Issue 3, pp. 331-8, 2000 (PubMed).