OB-Cadherin / Cadherin-11 antibody

Antigen: OB-Cadherin / Cadherin-11

Clonality: Monoclonal (16A)

Host: Mouse

Reactivity: Human, Rat (Rattus)

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335292

Additional Information

Immunogen: 16A is a mouse monoclonal IgG1 antibody obtained by fusion of SP2/0 mouse myeloma cells with spleen cells from a mouse immunized with affinity purified extracellular domain of human cadherin-11-GST fusion protein.

Format: Culture supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: 16A

Description: Cadherins constitute a family of transmembrane glycoproteins involved in Ca 2+ -dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers. The extracellular domains are responsible for the specificity of homophilic interactions between cells expressing the same cadherin. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is based on the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

Specificity: Human and rat.

Application Details

Application Notes: 16A recognizes the extracellular domain of cadherin-11. 16A is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen sections when using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl 2 . Optimal antibody dilution should be determined by titration,

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-OB-Cadherin / Cadherin-11 antibody

Publications

Publications: Bussemakers, Van Bokhoven, Tomita et al.: "Complex cadherin expression in human prostate cancer cells." in: International journal of cancer. Journal international du cancer, Vol. 85, Issue 3, pp. 446-50, 2000 (PubMed).

Tomita, van Bokhoven, van Leenders et al.: "Cadherin switching in human prostate cancer progression." in: Cancer research, Vol. 60, Issue 13, pp. 3650-4, 2000 (PubMed).