Cardiotin antibody

Antigen: Cardiotin

Clonality: Monoclonal (R2G)

Host: Mouse

Reactivity: Human, Monkey, Pig (Porcine), Cat (Feline), Dog (Canine), Goat, Hamster, Mouse (Murine), Rat (Rattus), Rabbit, Xenopus laevis

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335293

Additional Information

Immunogen: R2G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells, with spleen cells from a mouse immunized with a total protein extract of chicken gizzard.

Format: Culture supernatant

Isotype: IgM  (Matching secondary antibodies)

Clone: R2G

Description: Cardiotin is a high molecular weight protein complex (300 kDa) located in the mitochondria of cardiomyocytes and skeletal muscle. The cardiotin structure exists of subunits of 60 kDa and 100 kDa, probably in a tetrameric configuration. Both subunits contain the same amino-terminal 14 amino-acid sequence, showing high homology to human skeletal muscle alpha-actinin. During cardiac contractile dysfunction and myocard cell differentiation, the cardiotin distribution is affected. Compared to other structural proteins, cardiotin is one of the first to respond to insults (ischemia, fibrillation) that influence the functional status of cardiomyocytes.

Specificity: Human, monkey, swine, feline, canine, goat, hamster, mouse, rat, rabbit and Xenopus.

Application Details

Application Notes: R2G reacts with cardiotin, a mitochondrion-associated protein, which is present in cardiomyocytes and skeletal muscle. R2G is useful for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissue and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. In immunoblotting assays R2G reacts with the 300 kDa cardiotin protein complex and its 100 kDa and 60 kDa subunits. Optimal antibody dilution for immunoblotting applications should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Images

anti-Cardiotin antibody

anti-Cardiotin antibody (Image 2)

anti-Cardiotin antibody (Image 3)

Publications

Publications: Schaart, van der Ven, Ramaekers: "Characterization of cardiotin, a structural component in the myocard." in: European journal of cell biology, Vol. 62, Issue 1, pp. 34-48, 1994 (PubMed).

Schaart, Moens, Endert et al.: "Biochemical characterization of cardiotin, a sarcoplasmic reticulum associated protein." in: FEBS letters, Vol. 403, Issue 2, pp. 168-72, 1997 (PubMed).

Ausma, Wijffels, van Eys et al.: "Dedifferentiation of atrial cardiomyocytes as a result of chronic atrial fibrillation." in: The American journal of pathology, Vol. 151, Issue 4, pp. 985-97, 1997 (PubMed).

Dispersyn, Geuens, Ver Donck et al.: "Adult rabbit cardiomyocytes undergo hibernation-like dedifferentiation when co-cultured with cardiac fibroblasts." in: Cardiovascular research, Vol. 51, Issue 2, pp. 230-40, 2001 (PubMed).

Ausma, Litjens, Lenders et al.: "Time course of atrial fibrillation-induced cellular structural remodeling in atria of the goat." in: Journal of molecular and cellular cardiology, Vol. 33, Issue 12, pp. 2083-94, 2001 (PubMed).

Dispersyn, Mesotten, Meuris et al.: "Dissociation of cardiomyocyte apoptosis and dedifferentiation in infarct border zones." in: European heart journal, Vol. 23, Issue 11, pp. 849-57, 2002 (PubMed).

Ausma, van der Velden, Lenders et al.: "Reverse structural and gap-junctional remodeling after prolonged atrial fibrillation in the goat." in: Circulation, Vol. 107, Issue 15, pp. 2051-8, 2003 (PubMed).

Pochampally, Neville, Schwarz et al.: "Rat adult stem cells (marrow stromal cells) engraft and differentiate in chick embryos without evidence of cell fusion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9282-5, 2004 (PubMed).