Ovarian Carcinoma-Associated Antigen antibody

Antigen: Ovarian Carcinoma-Associated Antigen

Clonality: Monoclonal (OVTL16)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN335300

Additional Information

Immunogen: OVTL16 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cyst fluid, obtained from a patient with a poorly differentiated human ovarian carcinoma.

Format: Culture supernatant

Isotype: IgG1

Clone: OVTL16

Description: A central concept in tumor immunology is that malignancies bear tumor associated antigens, which are not or to a much lesser degree found in normal tissues. These antigens can be used as distinctive markers in diagnosis and therapy. As a consequence, monoclonal antibodies to such antigens may represent useful tools for histopathology, immunoscintigraphy and for immunotherapy .

Specificity: Human.

Application Details

Application Notes: OVTL16 recognizes a conformational epitope on ovarian carcinoma membrane antigen OA3 which is present in ovarian carcinomas and in cyst fluids obtained from these carcinomas, as well as in oviductal carcinomas and some endometrial carcinomas. OVTL16 is suitable for immunohistochemistry on frozen tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal antibody dilution should be determined by titration.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Research Area: Cancer

Restrictions: For Research Use only

Images

anti-Ovarian Carcinoma-Associated Antigen antibody

Publications

Publications: Boerman, Massuger, Makkink et al.: "Comparative in vitro binding characteristics and biodistribution in tumor-bearing athymic mice of anti-ovarian carcinoma monoclonal antibodies." in: Anticancer research, Vol. 10, Issue 5A, pp. 1289-95, 1990 (PubMed).

Boerman, Makkink, Massuger et al.: "Monoclonal antibodies against ovarian carcinoma-associated antigens, raised by immunization with cyst fluids." in: Anticancer research, Vol. 9, Issue 3, pp. 551-8, 1989 (PubMed).

Slobbe, Poels, ten Dam et al.: "Analysis of idiotope structure of ovarian cancer antibodies: recognition of the same epitope by two monoclonal antibodies differing mainly in their heavy chain variable sequences." in: Clinical and experimental immunology, Vol. 98, Issue 1, pp. 95-103, 1994 (PubMed).

Van Niekerk, Ramaekers, Hanselaar et al.: "Changes in expression of differentiation markers between normal ovarian cells and derived tumors." in: The American journal of pathology, Vol. 142, Issue 1, pp. 157-77, 1993 (PubMed).

van Niekerk, Vooijs, Casparie-van Velsen et al.: "Differentiation margins of ovarian tumor pathology: first incidences of epithelial ovarian tumors monitored by marker antibodies." in: Cancer detection and prevention, Vol. 21, Issue 3, pp. 247-57, 1997 (PubMed).