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Smoothelin antibody (SMTN)

Details for Product anti-SMTN Antibody No. ABIN335304, Supplier: Log in to see
Antigen
  • DKFZp468D019
  • MGC68765
  • MGC140000
  • MGC155237
  • smsmo
  • SMTN
Reactivity
Human, Dog (Canine), Cat (Feline), Chicken, Pig (Porcine), Monkey
19
5
3
3
3
3
3
2
1
1
Host
Mouse
15
9
Clonality (Clone)
Monoclonal ()
Conjugate
This Smoothelin antibody is un-conjugated
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
19
13
12
10
7
7
4
2
1
Supplier
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Immunogen R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal extract of chicken gizzard.
Clone R4A
Isotype IgG1
Specificity Human, canine, feline, chicken, swine, monkey. Not reactive with rat.
Purification Culture supernatant
Alternative Name Smoothelin (SMTN Antibody Abstract)
Background Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
Research Area Organogenesis, Cytoskeleton
Application Notes R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. For staining paraffin-embedded tissues pretreatment in 10 mM citrate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Can be diluted to 1:50.
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Supplier Images
 image for anti-Smoothelin antibody (SMTN) (ABIN335304) anti-Smoothelin (SMTN) antibody
 image for anti-Smoothelin antibody (SMTN) (ABIN335304) anti-Smoothelin (SMTN) antibody (Image 2)
Product cited in: Coco, Hirsch, Hornick: "Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract." in: The American journal of surgical pathology, Vol. 33, Issue 12, pp. 1795-801, 2009 (PubMed).

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, 2009 (PubMed).

Deruiter, Rensen, Coolen, Hierck, Bergwerff, Debie, Gittenberger-De Groot, Van Eys: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, 2001 (PubMed).

Johansson, Eriksson, Ramaekers, Thornell: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, 2000 (PubMed).

Johansson, Eriksson, Ramaekers, Thornell: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, 2000 (PubMed).

Christen, Bochaton-Piallat, Neuville, Rensen, Redard, van Eys, Gabbiani: "Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation." in: Circulation research, Vol. 85, Issue 1, pp. 99-107, 1999 (PubMed).

Wehrens, Mies, Gimona, Ramaekers, Van Eys, Small: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen, Ramaekers, van Eys: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).

van der Loop, Schaart, Timmer, Ramaekers, van Eys: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).