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Details for Product No. ABIN335304

Smoothelin (SMTN) antibody

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Antigen
Synonyms smsmo, MGC68765, SMTN, MGC140000, MGC155237, DKFZp468D019
Reactivity
»Alternatives Human, Dog (Canine), Cat (Feline), Chicken, Pig (Porcine), Monkey
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN335304
Quantity 1 mL
Price
264.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 5 to 7 Business Days
Immunogen R4A is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a cytoskeletal extract of chicken gizzard.
Clone R4A
Isotype IgG1
Specificity Human, canine, feline, chicken, swine, monkey. Not reactive with rat.
Purification Culture supernatant
Alternative Name Smoothelin
Background Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is located on chromosome 22.
Research Area Organogenesis, Cytoskeleton
Application Notes R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells. R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues and for staining with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. For staining paraffin-embedded tissues pretreatment in 10 mM citrate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Can be diluted to 1:50.
Restrictions For Research Use only
van der Loop, Schaart, Timmer et al.: "Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells." in: The Journal of cell biology, Vol. 134, Issue 2, pp. 401-11, 1996 (PubMed).

Wehrens, Mies, Gimona et al.: "Localization of smoothelin in avian smooth muscle and identification of a vascular-specific isoform." in: FEBS letters, Vol. 405, Issue 3, pp. 315-20, 1997 (PubMed).

van der Loop, Gabbiani, Kohnen et al.: "Differentiation of smooth muscle cells in human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 17, Issue 4, pp. 665-71, 1997 (PubMed).

Christen, Bochaton-Piallat, Neuville et al.: "Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation." in: Circulation research, Vol. 85, Issue 1, pp. 99-107, 1999 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin in adult and developing human arteries and myocardium." in: Histochemistry and cell biology, Vol. 112, Issue 4, pp. 291-9, 2000 (PubMed).

Johansson, Eriksson, Ramaekers et al.: "Smoothelin and intermediate filament proteins in human aortocoronary saphenous vein by-pass grafts." in: The Histochemical journal, Vol. 31, Issue 11, pp. 723-7, 2000 (PubMed).

Deruiter, Rensen, Coolen et al.: "Smoothelin expression during chicken embryogenesis: detection of an embryonic isoform." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 221, Issue 4, pp. 460-3, 2001 (PubMed).

Alternatives for antigen "Smoothelin (SMTN)", type "Antibodies"
Hosts (19)
Reactivities (17), (5), (3), (3), (3), (2), (2), (2), (2)
Applications (14), (9), (8), (7), (7), (7), (4), (3), (2)
Conjugates (2)
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