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Keratin 7 (KRT7) antibody

Details for Product No. ABIN335326, Supplier: Log in to see
Antigen
  • CK7
  • K2C7
  • K7
  • SCL
  • D15Wsu77e
  • Krt2-7
  • KRT7
  • keratin 7
  • KRT7
  • Krt7
Reactivity
Human, Mouse (Murine), Rat (Rattus), Hamster, Dog (Canine), Pig (Porcine), Cat (Feline), Zebrafish (Danio rerio)
514
145
77
22
12
10
10
7
6
4
2
1
1
1
1
1
Host
Mouse
359
203
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
19
13
12
11
11
9
9
9
9
9
9
9
9
9
9
9
7
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
329
270
252
148
133
81
75
69
28
26
18
13
8
8
7
3
2
Options
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Immunogen RCK105 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with cytokeratins from the human bladder carcinoma cell line T24.
Clone RCK105
Isotype IgG1
Specificity Human, mouse, rat, hamster, canine, swine and feline.
Purification Purified
Alternative Name Cytokeratin 7 / Keratin K7 (KRT7 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Application Notes RCK105 reacts exclusively with cytokeratin 7 which is present in a subgroup of glandular epithelia and their tumors, as well as transitional epithelium and transitional carcinoma. RCK105 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
 image for anti-Keratin 7 (KRT7) antibody (ABIN335326) anti-Keratin 7 (KRT7) antibody
Product cited in: Ogawa, Saeki, Igura, Hayashi: "Complementary expression and repulsive signaling suggest that EphB2 and ephrin-B1 are possibly involved in epithelial boundary formation at the squamocolumnar junction in the rodent stomach." in: Histochemistry and cell biology, 2013 (PubMed).

Bauwens, De Groot, Ramaekers, Veldman, Huizing: "Expression of intermediate filament proteins in the adult human vestibular labyrinth." in: The Annals of otology, rhinology, and laryngology, Vol. 101, Issue 6, pp. 479-86, 1992 (PubMed).

Smedts, Ramaekers, Troyanovsky, Pruszczynski, Link, Lane, Leigh, Schijf, Vooijs: "Keratin expression in cervical cancer." in: The American journal of pathology, Vol. 141, Issue 2, pp. 497-511, 1992 (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, 1992 (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, 1987 (PubMed).