Integrin alpha 6a antibody

Antigen: Integrin alpha 6a

Clonality: Monoclonal (1A10)

Host: Mouse

Reactivity: Human

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN335349

Additional Information

Immunogen: 1A10 is a mouse monoclonal IgG1, kappa antibody derived by fusion of SP2/0 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a synthetic peptide corresponding to the C-terminal 28 amino acids of integrin alpha6A including an appending N-terminal cysteine (CKKDHYDATYH-KAEIHAQPSDKERLTSDA) coupled to keyhole limpet hemocyanin.

Isotype: IgG1

Clone: 1A10

Description: Integrins are a family of heterodimeric membrane glycoproteins consisting of non-covalently associated alpha and beta subunits. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. In general, integrins function as receptors for extracellular matrix proteins. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. For integrin subunits alpha3 and alpha6, two cytoplasmic variants, A and B, have been identified.

Specificity: Human. A broad species reactivity is expected because of the conserved nature of the epitope.

Application Details

Application Notes: 1A10 reacts exclusively the cytoplasmic domain of non-phosphorylated integrin subunit alpha6A. 1A10 stains the basement membrane zone of skin, myoepithelial cells in sweat glands, peripheral nerves, sebaceous glands and some blood vessels in the dermis. 1A10 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:500 for immunoblotting applications.

Purification: Purified

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Publications

Hogervorst, Admiraal, Niessen et al.: "Biochemical characterization and tissue distribution of the A and B variants of the integrin alpha 6 subunit." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 179-91, 1993 (PubMed).