MUC1 antibody

Catalog No. ABIN335355

Product Details anti-MUC1 Antibody

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Specificity: Human.

Purification: Prepared from rabbit serum by affinity purification on a column made with the peptide antigen.

Immunogen: GP1.4 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with human milk fat globule.

Clone: GP1-4

Isotype: IgG1

Application Details

Application Notes: GP1.4 reacts with all glycoforms of Episialin. APPLICATIONS GP1.4 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin-embedded tissues, and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-MUC1 antibody (ABIN335355)

Norum, Sauren, Rye, Nustad: "New immunoassays for MUC1 in breast cancer." in: Tumour biology, Vol. 22, Issue 4, pp. 216-22, (2001) (PubMed).

Norum, Varaas, Kierulf, Nustad: "Carcinoma-associated MUC1 detected by immunoradiometric assays." in: Tumour biology, Vol. 19 Suppl 1, pp. 134-46, (1998) (PubMed).

Gourevitch, von Mensdorff-Pouilly, Litvinov, Kenemans, van Kamp, Verstraeten, Hilgers: "Polymorphic epithelial mucin (MUC-1)-containing circulating immune complexes in carcinoma patients." in: British journal of cancer, Vol. 72, Issue 4, pp. 934-8, (1995) (PubMed).

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: Episialin (MUC1 Products)

Synonyms: CA 15-3 antibody, CD227 antibody, EMA antibody, H23AG antibody, KL-6 antibody, MAM6 antibody, MCKD1 antibody, MUC-1 antibody, MUC-1/SEC antibody, MUC-1/X antibody, MUC1/ZD antibody, PEM antibody, PEMT antibody, PUM antibody, Muc-1 antibody, mucin 1, cell surface associated antibody, mucin 1, transmembrane antibody, MUC1 antibody, Muc1 antibody

Background: Episialin is also known as MUC1, MAM-6, CA 15-3, PEM and EMA. It is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. The gene encoding episialin (MUC1) is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. Episialin is present in several secretory epithelial cell types

Pathways: Negative Regulation of intrinsic apoptotic Signaling