CD56 antibody

Catalog No. ABIN335373

Product Details anti-CD56 Antibody

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD56 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Human and mouse.

Purification: Purified

Immunogen: RNL-1 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with the small cell lung cancer cell line NCI-H82.

Clone: RNL-1

Isotype: IgG1

Application Details

Application Notes: RNL-1 was defined as a cluster I antibody during the Second International Workshop on Small Cell Lung Cancer (SCLC) Antibodies. RNL-1 recognizes NCAM which is present in small cell lung cancer (SCLC) and lung carcinoids. RNL-1 is suitable for immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-CD56 antibody (ABIN335373)

Mahabir, Chatterjee, Misquitta, Chatterjee, Gerlai: "Lasting changes induced by mild alcohol exposure during embryonic development in BDNF, NCAM and synaptophysin-positive neurons quantified in adult zebrafish." in: The European journal of neuroscience, Vol. 47, Issue 12, pp. 1457-1473, (2018) (PubMed).

Hens, Nuydens, Geerts, Senden, Van de Ven, Roebroek, van de Velde, Ramaekers, Broers: "Neuronal differentiation is accompanied by NSP-C expression." in: Cell and tissue research, Vol. 292, Issue 2, pp. 229-37, (1998) (PubMed).

Kwa, Wesseling, Verhoeven, van Zandwijk, Hilkens: "Immunoscintigraphy of small-cell lung cancer xenografts with anti neural cell adhesion molecule monoclonal antibody, 123C3: improvement of tumour uptake by internalisation." in: British journal of cancer, Vol. 73, Issue 4, pp. 439-46, (1996) (PubMed).

Gerardy-Schahn, Eckhardt: "Hot spots of antigenicity in the neural cell adhesion molecule NCAM." in: International journal of cancer. Supplement = Journal international du cancer. Supplement, Vol. 8, pp. 38-42, (1994) (PubMed).

Broers, Mijnheere, Rot, Schaart, Sijlmans, Boerman, Ramaekers: "Novel antigens characteristic of neuroendocrine malignancies." in: Cancer, Vol. 67, Issue 3, pp. 619-33, (1991) (PubMed).

Boerman, Mijnheere, Broers, Vooijs, Ramaekers: "Biodistribution of a monoclonal antibody (RNL-1) against the neural cell adhesion molecule (NCAM) in athymic mice bearing human small-cell lung-cancer xenografts." in: International journal of cancer. Journal international du cancer, Vol. 48, Issue 3, pp. 457-62, (1991) (PubMed).

Mijnheere, Boerman, Broers, Klein Rot, Vooijs, Ramaekers: "Immunotargeting of human small cell lung cancer xenografts in athymic mice using a monoclonal antibody (RNL-1) against a neuroendocrine-related antigen." in: The British journal of cancer. Supplement, Vol. 14, pp. 78-81, (1991) (PubMed).

Moolenaar, Muller, Schol, Figdor, Bock, Bitter-Suermann, Michalides: "Expression of neural cell adhesion molecule-related sialoglycoprotein in small cell lung cancer and neuroblastoma cell lines H69 and CHP-212." in: Cancer research, Vol. 50, Issue 4, pp. 1102-6, (1990) (PubMed).

Langdon, Lawrie, Hay, Hawkes, McDonald, Hayward, Schol, Hilgers, Leonard, Smyth: "Characterization and properties of nine human ovarian adenocarcinoma cell lines." in: Cancer research, Vol. 48, Issue 21, pp. 6166-72, (1988) (PubMed).

Target Details for CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Alternative Name: NCAM / CD56 (NCAM1 Products)

Synonyms: CD56 antibody, MSK39 antibody, NCAM antibody, fj56g09 antibody, ncam antibody, ncam1 antibody, wu:fj56g09 antibody, NCAM1 antibody, n-cam antibody, E-NCAM antibody, NCAM-1 antibody, Ncam antibody, Cd56 antibody, N-CAM antibody, N-CAM-1 antibody, NCAM-C antibody, NCAMC antibody, N-CAM-1-B antibody, NCAM-1-B antibody, ncam1-a antibody, ncam1-b antibody, neural cell adhesion molecule 1 antibody, neural cell adhesion molecule 1a antibody, neural cell adhesion molecule 1 S homeolog antibody, neural cell adhesion molecule 1 L homeolog antibody, NCAM1 antibody, ncam1a antibody, ncam1 antibody, LOC100085373 antibody, ncam1.S antibody, Ncam1 antibody, ncam1.L antibody

Background: NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins.