Nestin antibody

Catalog No. ABIN335378

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: 10C2 reacts exclusively with the human nestin protein. No cross-reactivity to rodent nestin.

Purification: Purified

Immunogen: Nestin (clone 10C2) is a monoclonal antibody derived by traditional hybridoma technology. A 150-amino-acid fragment from the cloned human nestin was conjugated to glutathione S-transferase and used as an immunogen.

Clone: 10C2

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Storage: -20 °C

References for anti-Nestin antibody (ABIN335378)

Sellheyer, Krahl: "Does the peritumoral stroma of basal cell carcinoma recapitulate the follicular connective tissue sheath?" in: Journal of cutaneous pathology, Vol. 38, Issue 7, pp. 551-9, (2011) (PubMed).

Elamin, Shinwari, Hendrayani, Al-Hindi, Al-Shail, Khafaga, Al-Kofide, Aboussekhra: "Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells." in: Molecular carcinogenesis, Vol. 49, Issue 3, pp. 302-14, (2010) (PubMed).

Popescu, Manole, Gherghiceanu, Ardelean, Nicolescu, Hinescu, Kostin: "Telocytes in human epicardium." in: Journal of cellular and molecular medicine, Vol. 14, Issue 8, pp. 2085-93, (2010) (PubMed).

Sellheyer, Krahl et al.: "Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult ..." in: Journal of the American Academy of Dermatology, Vol. 63, Issue 1, pp. 93-113, (2010) (PubMed).

Murphy, Viero, Ho, Thorner: "Diagnostic utility of nestin expression in pediatric tumors in the region of the kidney." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 17, Issue 6, pp. 517-23, (2009) (PubMed).

Mokry, Pudil, Ehrmann, Cizkova, Osterreicher, Filip, Kolar: "Re-expression of nestin in the myocardium of postinfarcted patients." in: Virchows Archiv : an international journal of pathology, Vol. 453, Issue 1, pp. 33-41, (2008) (PubMed).

Corsino, Davis, Nørgaard, Parker, Law, Dunn, Law: "Mammary tumors initiated by constitutive Cdk2 activation contain an invasive basal-like component." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 11, pp. 1240-52, (2008) (PubMed).

Hou, Tan, Xu, Wang, Lu, Ji, Wang, Zhu: "Schwannoma of the gastrointestinal tract: a clinicopathological, immunohistochemical and ultrastructural study of 33 cases." in: Histopathology, Vol. 48, Issue 5, pp. 536-45, (2006) (PubMed).

Messam, Hou, Major: "Coexpression of nestin in neural and glial cells in the developing human CNS defined by a human-specific anti-nestin antibody." in: Experimental neurology, Vol. 161, Issue 2, pp. 585-96, (2000) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Background: Nestin is a Class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.