Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Vimentin antibody

VIM Reactivity: Human, Mouse, Rat, Chicken, Monkey, Dog, Goat, Hamster, Zebrafish (Danio rerio) WB, IHC, FACS, ICC, IHC (fro) Host: Mouse Monoclonal RV202 unconjugated
Catalog No. ABIN335380
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Reactivity
    • 332
    • 125
    • 124
    • 61
    • 58
    • 42
    • 41
    • 35
    • 29
    • 20
    • 20
    • 13
    • 12
    • 8
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Chicken, Monkey, Dog, Goat, Hamster, Zebrafish (Danio rerio)
    Host
    • 190
    • 128
    • 9
    • 6
    • 4
    • 4
    • 2
    Mouse
    Clonality
    • 198
    • 144
    Monoclonal
    Conjugate
    • 199
    • 19
    • 13
    • 13
    • 11
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 281
    • 131
    • 99
    • 97
    • 96
    • 65
    • 48
    • 40
    • 39
    • 27
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Human, mouse, rat, hamster, chicken, goat, canine and monkey.
    Purification
    Purified
    Immunogen
    RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.
    Clone
    RV202
    Isotype
    IgG1
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes
    RV202 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

    Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

    Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

    Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." in: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

    Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." in: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

  • Target
    Vimentin (VIM)
    Alternative Name
    Vimentin (VIM Products)
    Synonyms
    CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
    Background
    Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
    Pathways
    Caspase Cascade in Apoptosis
You are here:
Support