Mitotic Cells antibody

Catalog No. ABIN335393

Product Details

Target: Mitotic Cells

Reactivity: Human, Zebrafish (Danio rerio)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: Human.

Purification: Purified

Immunogen: 8B3G is a mouse monoclonal IgM antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a mouse immunized with a total cell lysate of the human bladder carcinoma cell line T24.

Clone: 8B3G

Isotype: IgM

Application Details

Application Notes: 8B3G strongly stains mitotic cells and can therefore be used in flow cytometric analyses of cell suspensions to detect the mitotic index. Together with a quantitative DNA staining procedure (e.g. propidium iodide) 8B3G clearly distinguishes these M-phase cells from cell at other stages of the cell cycle (see figure). Dynamic information can be obtained by combining BrdU incorporation with 8B3G staining, which can distinguish and quantitate the four major fractions of the cell cycle. 8B3G can be used for flow cytometric analyses and immunocytochemistry. 8B3G is not suitable for immunoblotting. Optimal antibody dilution should be determined by titration, recommended range is 1:50 - 1:100 for flow cytometry, and for immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Dual parameter flow cytometric analysis of human colon cancer HT29 cells stained with monoclonal antibody 8B3G and propidium iodide (PI). The mitotic cell fraction is encircled.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Target Details

Target: Mitotic Cells

Background: The life cycle of a eukaryotic cell consists of various phases, two of which can morphologically and biochemically be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques, such as the BrdU incorporation assay. In between the M- and S-phase two gap phases occur: the G 1 -phase, the gap between mitosis and the start of DNA replication, and G 2 -phase, the gap between completion of DNA replication and the onset of mitosis. From G 1 -phase a cell can leave the cell cycle and enter G 0 , a €˜quiescent€™ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G 0 to G 1 , from G 1 to S, and from G 2 to M-phase.