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EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN335407, Supplier: Log in to see
Antigen
  • sb:cb6
  • tacstd
  • wu:fj17g02
  • zgc:110304
  • zgc:77119
  • tacstd1
  • MGC80540
  • EPCAM
  • TACSTD1
  • egp
  • ksa
  • m4s1
  • mk-1
  • cd326
  • egp40
  • mic18
  • trop1
  • ep-cam
  • hegp-2
  • co17-1a
  • ga733-2
  • GP40
  • LEU-9
  • TP41
  • Tp40
  • DIAR5
  • EGP-2
  • EGP314
  • EGP40
  • ESA
  • HNPCC8
  • KS1/4
  • KSA
  • M4S1
  • MIC18
  • MK-1
  • TROP1
  • CD326
  • EGP
  • Egp314
  • Ep-CAM
  • EpCAM1
  • GA733-2
  • Ly74
  • Tacsd1
  • Tacstd1
  • gp40
Reactivity
Human
439
91
45
2
1
1
1
Host
Mouse
335
127
39
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
34
23
16
13
6
5
5
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
288
211
130
120
118
117
68
49
38
13
8
5
5
4
4
4
2
2
2
1
1
1
1
1
Supplier
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Immunogen VU1D9 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with native Ep-CAM from lung tissue.
Clone VU1D9
Isotype IgG1
Specificity Human.
Purification Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
Alternative Name Ep-CAM (EPCAM Antibody Abstract)
Background Ep-CAM is a 40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca 2+ -independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.
Pathways
Application Notes VU1D9 reacts with the first EGF repeat in the extracellular domain of Ep-CAM. The epitope is sensitive for reducing agents. VU1D9 detects Ep-CAM in most simple, pseudostratified and transitional epithelia and in the majority of human carcinomas. APPLICATIONS VU1D9 is suitable for immunoblotting (non-reducing conditions), immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Product cited in: Balzar, Briaire-de Bruijn, Rees-Bakker et al.: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, 2001 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).

Litvinov, Velders, Bakker et al.: "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 437-46, 1994 (PubMed).

Tsubura, Senzaki, Sasaki et al.: "Immunohistochemical demonstration of breast-derived and/or carcinoma-associated glycoproteins in normal skin appendages and their tumors." in: Journal of cutaneous pathology, Vol. 19, Issue 1, pp. 73-9, 1992 (PubMed).