Epithelial Cell Adhesion Molecule (EPCAM) antibody

Antigen: Epithelial Cell Adhesion Molecule (EPCAM)

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Clonality: Monoclonal (VU1D9)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN335407

Additional Information

Alternative name: Ep-CAM

Immunogen: VU1D9 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with native Ep-CAM from lung tissue.

Isotype: IgG1

Clone: VU1D9

Description: Ep-CAM is a 40 kD glycoprotein, which functions as an epithelial cell adhesion molecule. Ep-CAM mediates Ca 2+ -independent homotypic interactions. Formation of Ep-CAM-mediated adhesions has a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is expressed in the majority of human carcinomas and is a marker for tumors of epithelial lineage.

Specificity: Human.

Synonyms: EGP, ESA, KSA, M4S1, MK-1, EGP-2, EGP34, EGP40, KS1/4, MIC18, TROP1, CO-17A, Ep-CAM, hEGP-2, CO17-1A, GA733-2, TACST-1, TACSTD1, sb:cb6, tacstd, zgc:77119, wu:fj17g02, EPCAM, tacstd1, MGC80540, MGC127627, egp, ksa, m4s1, mk-1, cd326, egp40, mic18, trop1, ep-cam, hegp-2, co17-1a, ga733-2

Application Details

Application Notes: VU1D9 reacts with the first EGF repeat in the extracellular domain of Ep-CAM. The epitope is sensitive for reducing agents. VU1D9 detects Ep-CAM in most simple, pseudostratified and transitional epithelia and in the majority of human carcinomas. APPLICATIONS VU1D9 is suitable for immunoblotting (non-reducing conditions), immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Storage: Store at 4 o C, or in small aliquots at -20 o C.

Restrictions: For Research Use only

Publications

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).